Complications and pitfalls in rat stroke models for middle cerebral artery occlusion: a comparison between the suture and the macrosphere model using magnetic resonance angiography
Department of Neurology
Animals; Carotid Artery, External; *Disease Models, Animal; *Infarction, Middle Cerebral Artery; Ligation; Magnetic Resonance Angiography; Magnetic Resonance Imaging; Male; Microspheres; Rats; Rats, Sprague-Dawley; Suture Techniques; Titanium
Molecular and Cellular Neuroscience | Neurology | Neuroscience and Neurobiology
BACKGROUND AND PURPOSE: Investigating focal cerebral ischemia requires animal models that are relevant to human stroke. Complications and side effects are common among these models. The present study describes potential pitfalls in 3 techniques for middle cerebral artery occlusion (MCAO) in rats using magnetic resonance imaging (MRI) and magnetic resonance angiography (MRA).
METHODS: Rats were subjected to temporary MCAO for 90 minutes using the suture technique (group I; n=10) or to permanent MCAO using the suture technique (group II; n=10) or the macrosphere technique (group III; n=10). Clinical evaluation was performed after 3 hours and 24 hours. After 24 hours, animals underwent MRI and MRA to determine lesion size and the intracranial vascular status.
RESULTS: Hemispheric lesion volume was significantly smaller in group I (14.6%) compared with groups II (35.2%; PCONCLUSIONS: Model failures occurred frequently in all groups. MRI and MRA helps to identify animals that need to be excluded from experimental stroke studies.
DOI of Published Version
Stroke. 2004 Oct;35(10):2372-7. Epub 2004 Sep 2. Link to article on publisher's site
Stroke; a journal of cerebral circulation
Gerriets T, Stolz E, Walberer M, Muller C, Rottger C, Kluge A, Kaps M, Fisher M, Bachmann G. (2004). Complications and pitfalls in rat stroke models for middle cerebral artery occlusion: a comparison between the suture and the macrosphere model using magnetic resonance angiography. Neurology Publications and Presentations. https://doi.org/10.1161/01.STR.0000142134.37512.a7. Retrieved from https://escholarship.umassmed.edu/neuro_pp/58