Complications and pitfalls in rat stroke models for middle cerebral artery occlusion: a comparison between the suture and the macrosphere model using magnetic resonance angiography
Department of Neurology
Animals; Carotid Artery, External; *Disease Models, Animal; *Infarction, Middle Cerebral Artery; Ligation; Magnetic Resonance Angiography; Magnetic Resonance Imaging; Male; Microspheres; Rats; Rats, Sprague-Dawley; Suture Techniques; Titanium
Molecular and Cellular Neuroscience | Neurology | Neuroscience and Neurobiology
BACKGROUND AND PURPOSE: Investigating focal cerebral ischemia requires animal models that are relevant to human stroke. Complications and side effects are common among these models. The present study describes potential pitfalls in 3 techniques for middle cerebral artery occlusion (MCAO) in rats using magnetic resonance imaging (MRI) and magnetic resonance angiography (MRA).
METHODS: Rats were subjected to temporary MCAO for 90 minutes using the suture technique (group I; n=10) or to permanent MCAO using the suture technique (group II; n=10) or the macrosphere technique (group III; n=10). Clinical evaluation was performed after 3 hours and 24 hours. After 24 hours, animals underwent MRI and MRA to determine lesion size and the intracranial vascular status.
RESULTS: Hemispheric lesion volume was significantly smaller in group I (14.6%) compared with groups II (35.2%; PCONCLUSIONS: Model failures occurred frequently in all groups. MRI and MRA helps to identify animals that need to be excluded from experimental stroke studies.
DOI of Published Version
Stroke. 2004 Oct;35(10):2372-7. Epub 2004 Sep 2. Link to article on publisher's site
Stroke; a journal of cerebral circulation
Gerriets, Tibo; Stolz, Erwin; Walberer, Maureen; Muller, Clemens; Rottger, Carina; Kluge, Alexander; Kaps, Manfred; Fisher, Marc; and Bachmann, Georg, "Complications and pitfalls in rat stroke models for middle cerebral artery occlusion: a comparison between the suture and the macrosphere model using magnetic resonance angiography" (2004). Neurology Publications and Presentations. 58.