Title
Regional variations in the apparent diffusion coefficient and the intracellular distribution of water in rat brain during acute focal ischemia
UMMS Affiliation
Department of Neurology; Department of Radiology; Graduate School of Biomedical Sciences
Publication Date
2001-8
Document Type
Article
Subjects
Acute Disease; Animals; Astrocytes; Brain; Brain Chemistry; Brain Edema; Brain Ischemia; Capillaries; Cell Compartmentation; Cell Size; Diffusion; Disease Models, Animal; Intracellular Fluid; Magnetic Resonance Imaging; Neurons; Neuropil; Rats; Rats, Sprague-Dawley; Water
Disciplines
Nervous System Diseases | Neurology
Abstract
BACKGROUND AND PURPOSE: The apparent diffusion coefficient of water (ADC) rapidly drops in ischemic tissue after cerebral artery occlusion. This acute drop is thought to be caused by the loss of extracellular fluid and the gain of intracellular fluid. To test the latter possibility, changes in ADC and the size of several cellular compartments were assessed in 3 regions of rat brain at the end of 90 minutes of focal cerebral ischemia.
METHODS: One middle cerebral artery was permanently occluded in 8 Sprague-Dawley rats; sham occlusions were performed in 2 other rats. ADC maps were generated 90 minutes later, and the brains were immediately perfusion fixed. Three regions of interest (ROIs) were defined on the basis of ADC range. Various neuronal, astrocytic, and capillary compartments in each ROI were quantified with light and electron microscopy.
RESULTS: At the end of 90 minutes of ischemia, mean ADC was normal in the cortex of sham-operated rats and the contralateral cortex of ischemic rats (ROI-a), 25% lower in the ipsilateral frontoparietal cortex (ROI-b), and 45% lower in the ischemic lateral caudoputamen (ROI-c). At this time, the frequency of swollen astrocytic cell bodies and volume of swollen dendrites and astrocytic processes in neuropil were ROI-a
Source
Stroke. 2001 Aug;32(8):1897-905.
Journal/Book/Conference Title
Stroke; a journal of cerebral circulation
Related Resources
PubMed ID
11486123
Repository Citation
Liu, Kai-Feng; Li, Fuhai; Tatlisumak, Turgut; Garcia, Julio H.; Sotak, Christopher H.; Fisher, Marc; and Fenstermacher, Joseph D., "Regional variations in the apparent diffusion coefficient and the intracellular distribution of water in rat brain during acute focal ischemia" (2001). Neurology Publications and Presentations. 51.
https://escholarship.umassmed.edu/neuro_pp/51