Department of Neurology
Animals; Brain; Cells, Cultured; DEAD-box RNA Helicases; Female; *Fragile X Mental Retardation Protein; Fragile X Syndrome; HEK293 Cells; Humans; Male; Mice; Mice, Knockout; MicroRNAs; RNA-Binding Proteins; Ribonuclease III
Neurology | Neuroscience and Neurobiology
Mammalian brain-specific miR-9 and miR-124 have been implicated in several aspects of neuronal development and function. However, it is not known how their expression levels are regulated in vivo. We found that the levels of miR-9 and miR-124 are regulated by FXR1P but not by the loss of FXR2P or FMRP in vivo, a mouse model of fragile X syndrome. Surprisingly, the levels of miR-9 and miR-124 are elevated in fmr1/fxr2 double-knock-out mice, in part reflecting posttranscriptional upregulation of FXR1P. Indeed, FXR1P is required for efficient processing of pre-miR-9 and pre-miR-124 in vitro and forms a complex with Dicer and pre-miRNAs. These findings reveal differential roles of FMRP family proteins in controlling the expression levels of brain-specific miRNAs.
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DOI of Published Version
J Neurosci. 2011 Sep 28;31(39):13705-9. Link to article on publisher's site
The Journal of neuroscience : the official journal of the Society for Neuroscience
Xu, Xia-Lian; Zong, Ruiting; Li, Zhaodong; Biswas, Md Helal Uddin; Fang, Zhe; Nelson, David L.; and Gao, Fen-Biao, "FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124" (2011). Neurology Publications and Presentations. 412.