Title

Embryonic stem cell rescue of tremor and ataxia in myelin-deficient shiverer mice

UMMS Affiliation

Department of Neurology; Department of Cell Biology

Publication Date

2009-1

Document Type

Article

Subjects

Stem cells; Myelin basic protein; *X-gal histochemistry; Blastocyst injection; Chimera; Oligodendrocytes; Shiverer mice; White matter

Disciplines

Cell Biology | Neurology | Neuroscience and Neurobiology

Abstract

Transplantation of neural precursor cells has been proposed as a possible approach for replacing missing or damaged central nervous system myelin. Neonatal and adult myelin-deficient shiverer (shi) mice, bearing a mutation of the myelin basic protein (MBP) gene, have been used extensively as hosts for testing cell engraftment, migration, and myelination, but relatively little progress has been made in reversing shi motor deficits. Here we describe a prenatal cell replacement strategy, showing that embryonic stem cells injected into shi blastocyst embryos can generate chimeric mice with strong and widespread immunoreactive MBP expression throughout the brain and a behavioral (motor) phenotype that appears essentially rescued.

DOI of Published Version

10.1016/j.jns.2008.09.037

Source

J Neurol Sci. 2009 Jan 15;276(1-2):133-7. Epub 2008 Nov 8. Link to article on publisher's site

Journal/Book/Conference Title

Journal of the neurological sciences

Related Resources

Link to Article in PubMed

PubMed ID

18996543

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