Neuronal differentiation of EGF-propagated neurosphere cells after engraftment to the nucleus of the solitary tract

UMMS Affiliation

Department of Neurology



Document Type



Animals; Animals, Newborn; Cell Differentiation; Culture Media; Embryonic Stem Cells; Epidermal Growth Factor; Injections, Spinal; Mice; Mice, Transgenic; Neurons; Solitary Nucleus


Molecular and Cellular Neuroscience | Neuroscience and Neurobiology


Neural precursor cells expanded with epidermal growth factor (EGF) exhibit multipotentiality in vitro, but they differentiate predominantly as glial phenotypes after their transplantation in vivo. Here we demonstrate that EGF-propagated precursors from the murine striatal subventricular zone can exhibit robust incorporation and neuronal differentiation within the nucleus of the solitary tract (NST) after injection into the cisterna magna of neonatal or young adult mice. About two-third of engrafted cells appeared NeuN positive in the region of the gelatinous subnucleus, a region notable for its lack of myelinated fibers. The NST may provide a useful model for understanding the physiological and metabolic regulation of postnatal neurogenesis.

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Citation: Neurosci Lett. 2008 Oct 31;444(3):250-3. Epub 2008 Aug 22. Link to article on publisher's site

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