Visualization of cortical spreading depression using manganese-enhanced magnetic resonance imaging

UMMS Affiliation

Department of Neurology; Department of Radiology; Graduate School of Biomedical Sciences

Publication Date


Document Type



Animals; Image Processing, Computer-Assisted; Magnetic Resonance Imaging; Male; Manganese; Potassium Chloride; Rats; Rats, Sprague-Dawley; Reproducibility of Results; *Spreading Cortical Depression


Neurology | Radiology


Cortical spreading depression (CSD) was visualized using manganese-enhanced MRI (MEMRI) following topical application of KCl to the exposed rat cortex. MEMRI signal increase in the ipsilateral cortex relative to the contralateral control region was 60 +/- 30% following two KCl applications. MEMRI signal increase for a single (40%) versus double (80%) KCl application suggests an integration effect over successive CSD episodes. CSD-induced MEMRI enhancement involved cortical layers containing dense regions of apical dendrites, supporting the contention that these neuronal structures are necessary for propagation of CSDs. Subcortical enhancement was present in hippocampal and thalamic regions, most likely a result of neuronal connections with cortical layers 4 and 5. These results are consistent with previous studies of CSD using diffusion-weighted MRI and T(2) (*)-weighted MRI and should be useful for investigating CSD itself and its role in other neurologic disorders.

DOI of Published Version



Magn Reson Med. 2005 Apr;53(4):851-7. Link to article on publisher's site

Journal/Book/Conference Title

Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine

Related Resources

Link to article in PubMed

PubMed ID