Title
Effects of intravenous dimethyl sulfoxide on ischemia evolution in a rat permanent occlusion model
UMMS Affiliation
Department of Neurology
Publication Date
2005-03-04
Document Type
Article
Subjects
Animals; Behavior, Animal; Blood Pressure; Body Temperature; Brain; Brain Ischemia; Cerebrovascular Circulation; Diffusion Magnetic Resonance Imaging; Dimethyl Sulfoxide; Disease Progression; Functional Laterality; Heart Rate; Infarction, Middle Cerebral Artery; Injections, Intravenous; Male; *Neuroprotective Agents; Rats; Rats, Inbred WKY
Disciplines
Nervous System Diseases | Neurology
Abstract
Dimethyl sulfoxide (DMSO) has a variety of biological actions that suggest efficacy as a neuroprotectant. We (1) tested the neuroprotective potential of DMSO at different time windows on infarct size using 2,3,5-triphenyltetrazolium staining and (2) investigated the effects of DMSO on ischemia evolution using quantitative diffusion and perfusion imaging in a permanent middle cerebral artery occlusion (MCAO) model in rats. In experiment 1, DMSO treatment (1.5 g/kg intravenously over 3 h) reduced infarct volume 24 h after MCAO by 65% (P<0.00001) when initiated 20 h before MCAO, by 44% (P=0.0006) when initiated 1 h after MCAO, and by 17% (P=0.11) when started 2 h after MCAO. Significant infarct reduction was also observed after a 3-day survival in animals treated 1 h after MCAO (P=0.005). In experiment 2, treatment was initiated 1 h after MCAO and maps for cerebral blood flow (CBF) and apparent diffusion coefficient (ADC) were acquired before treatment and then every 30 mins up to 4 h. Cerebral blood flow characteristics and CBF-derived lesion volumes did not differ between treated and untreated animals, whereas the ADC-derived lesion volume essentially stopped progressing during DMSO treatment, resulting in a persistent diffusion/perfusion mismatch. This effect was mainly observed in the cortex. Our data suggest that DMSO represents an interesting candidate for acute stroke treatment.
DOI of Published Version
10.1038/sj.jcbfm.9600095
Source
J Cereb Blood Flow Metab. 2005 Aug;25(8):968-77. Link to article on publisher's site
Journal/Book/Conference Title
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
Related Resources
PubMed ID
15744247
Repository Citation
Bardutzky JF, Meng X, Bouley JP, Duong TQ, Ratan R, Fisher M. (2005). Effects of intravenous dimethyl sulfoxide on ischemia evolution in a rat permanent occlusion model. Neurology Publications. https://doi.org/10.1038/sj.jcbfm.9600095. Retrieved from https://escholarship.umassmed.edu/neuro_pp/174