The role of spreading depression in focal ischemia evaluated by diffusion mapping
Authors
Takano, KentaroLatour, Lawrence L.
Formato, James E.
Carano, Richard A.D.
Helmer, Karl G.
Hasegawa, Yasuhiro
Sotak, Christopher H.
Fisher, Marc
UMass Chan Affiliations
Graduate School of Biomedical SciencesDepartment of Radiology
Department of Neurology
Document Type
Journal ArticlePublication Date
1996-03-01Keywords
AnimalsBrain Ischemia
Cerebral Arteries
Magnetic Resonance Imaging
Male
Rats
Rats, Sprague-Dawley
Spreading Cortical Depression
Stroke Volume
Time Factors
Molecular and Cellular Neuroscience
Neuroscience and Neurobiology
Metadata
Show full item recordAbstract
This study investigated the role of spontaneous and induced spreading depression (SD) on the evolution of focal ischemia in vivo. We induced focal ischemia in 12 rats using the middle cerebral artery suture occlusion (MCAO) method. Chemical stimulation of nonischemic ipsilateral cortex by potassium chloride application (KCl group; n = 7) and saline (NaCl group; n = 5) was performed at 15, 30, 45, and 60 minutes following MCAO, and SD was detected electrophysiologically. Ischemic lesion volumes assessed over 15-minute intervals, evaluated by continuous apparent diffusion coefficient (ADC) of water mapping, demonstrated that the ischemic region increased significantly during 15-minute time epochs with a single SD episode (36.5 +/- 12.9 mm3, mean +/- SD) or multiple SD episodes (39.8 +/- 22.3) compared with those without SD (13.9 +/- 11.5) (p = 0.0009). Infarct volume at postmortem 24 hours after MCAO was significantly larger in the KCl group, with more total SDs (237.8 +/- 13.8) than the NaCl group (190.5 +/- 12.6) (p = 0.0001). This study demonstrates that ischemia-related and induced SDs increase significantly ischemic lesion volume in vivo, supporting the hypothesis for a causative role of SD in extending focal ischemic injury.Source
Ann Neurol. 1996 Mar;39(3):308-18. Link to article on publisher's siteDOI
10.1002/ana.410390307Permanent Link to this Item
http://hdl.handle.net/20.500.14038/37621PubMed ID
8602749Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/ana.410390307