Determination of focal ischemic lesion volume in the rat brain using multispectral analysis

UMMS Affiliation

Department of Neurology; Department of Radiology; Graduate School of Biomedical Sciences

Publication Date


Document Type



Algorithms; Animals; Brain; Brain Ischemia; Disease Models, Animal; Echo-Planar Imaging; Image Processing, Computer-Assisted; *Magnetic Resonance Spectroscopy; Male; Rats; Rats, Sprague-Dawley


Molecular and Cellular Neuroscience | Neuroscience and Neurobiology | Radiology


Multispectral (MS) analysis was used to determine the ischemic lesion volume in the rat brain after permanent middle cerebral arterial occlusion. MS analysis used a four-dimensional MS model consisting of an estimate of the average apparent diffusion coefficient of water (ADC(av)), T2, proton density, and perfusion. Four classification methods were investigated: (a) multivariate gaussian (MVG); (b) k-nearest neighbor (k-NN); (c) k-means (KM); and (d) fuzzy c-means (FCM). MVG and k-NN classifiers are supervised methods requiring labeled training data to characterize the stroke lesion. Unsupervised classifiers (KM, FCM) do not require previous statistics or labeled training data, resulting in potentially greater clinical usefulness. All MS methods provided significant correlation with postmortem findings beyond the use of ADC(av) alone (partial correlation given the ADC(av) estimate: MVG, .66; k-NN, .75; KM, .68; FCM, .70). This study demonstrates that MS analysis provides an improved estimate of ischemic lesion volume over that obtained from ADC alone.


J Magn Reson Imaging. 1998 Nov-Dec;8(6):1266-78.

Journal/Book/Conference Title

Journal of magnetic resonance imaging : JMRI

Related Resources

Link to article in PubMed

PubMed ID