Title
Investigation of techniques to quantify in vivo lesion volume based on comparison of water apparent diffusion coefficient (ADC) maps with histology in focal cerebral ischemia of rats
UMMS Affiliation
Department of Neurology; Department of Radiology; Graduate School of Biomedical Sciences
Publication Date
2004-06-03
Document Type
Article
Subjects
Acute Disease; Animals; Brain Ischemia; Diffusion Magnetic Resonance Imaging; Male; Middle Cerebral Artery; Models, Animal; Rats; Rats, Sprague-Dawley; Time Factors
Disciplines
Neurology | Radiology
Abstract
Stroke lesion-volume estimates derived from calculated water apparent diffusion coefficient (ADC) maps provide a quantitative surrogate end-point for investigating the efficacy of drug treatment or studying the temporal evolution of cerebral ischemia. Methodology is described for estimating ischemic lesion volumes in a rat model of permanent middle cerebral artery occlusion (MCAO) based on absolute and percent-reduction threshold values of the water ADC at 3 h post-MCAO. Volume estimates derived from average ADC (ADC(av)) maps were compared with those derived from post-mortem histological sections. Optimum ADC thresholds were established as those that provided the best correlation and one-to-one correspondence between ADC- and histologically derived lesion-volume estimates. At 3 h post-MCAO, an absolute-ADC(av) threshold of 47 x 10(-5) mm(2)/s (corresponding to a 33% reduction in ADC(av) based on a contralateral hemisphere comparison) provided the most accurate estimate of percent hemispheric lesion volume (%HLV). Experimental and data analysis issues for improving and validating the usefulness of DWI as a surrogate endpoint for the quantification of ischemic lesion volume are discussed.
DOI of Published Version
10.1016/j.mri.2004.01.043
Source
Magn Reson Imaging. 2004 Jun;22(5):653-9. Link to article on publisher's site
Journal/Book/Conference Title
Magnetic resonance imaging
Related Resources
PubMed ID
15172059
Repository Citation
Kazemi M, Silva MD, Li F, Fisher M, Sotak CH. (2004). Investigation of techniques to quantify in vivo lesion volume based on comparison of water apparent diffusion coefficient (ADC) maps with histology in focal cerebral ischemia of rats. Neurology Publications and Presentations. https://doi.org/10.1016/j.mri.2004.01.043. Retrieved from https://escholarship.umassmed.edu/neuro_pp/152