Temperature dependent change of apparent diffusion coefficient of water in normal and ischemic brain of rats

UMMS Affiliation

Department of Neurology; Department of Radiology; Graduate School of Biomedical Sciences

Publication Date


Document Type



Animals; *Body Temperature; Brain Ischemia; Diffusion; Feasibility Studies; Magnetic Resonance Imaging; Male; Rats; Rats, Sprague-Dawley; Reference Values; Thermometers; Water


Bioimaging and Biomedical Optics | Molecular and Cellular Neuroscience | Neurology


To identify the temperature dependent change of the apparent diffusion coefficient (ADC) of water in brain tissue, the ADC values of normal rat brain were measured over a range of body temperatures with monitoring of head temperature using a small water reference implanted under the temporalis muscle. An initial experiment using thermocouples implanted into the cortex, caudate-putamen, temporalis muscle, and rectum demonstrated that temperature in all regions were highly correlated over a temperature range from 33 to 39 degrees C. In another group of normal rats, brain ADC values varied almost uniformly with body temperature over the temperature range 33-39 degrees C, implying that brain ADC values accurately reflect changes in brain temperature. The effects of focal ischemia and administration of the noncompetitive N-methyl-D-aspartate (NMDA) antagonist, CNS-1102, on ADC were also examined, using the suture middle cerebral artery (MCA) occlusion model while maintaining the body temperature at 37 degrees C. ADC values and therefore brain temperature in the nonischemic and ischemic hemispheres were not affected by the drug. These experiments suggest that brain ADC measurement could be useful in animal studies and, potentially, in humans to assess the effects of pharmacologic intervention on brain temperature.


J Cereb Blood Flow Metab. 1994 May;14(3):383-90.

Journal/Book/Conference Title

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

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Link to article in PubMed

PubMed ID