Reproducibility and reliability of middle cerebral artery occlusion using a silicone-coated suture (Koizumi) in rats

UMMS Affiliation

Department of Neurology

Publication Date


Document Type



Animals; Brain Ischemia; Cerebral Arteries; Disease Models, Animal; Male; Rats; Rats, Sprague-Dawley; Silicones; *Sutures


Nervous System Diseases | Neurology


The best technical approach to rat middle cerebral artery occlusion (MCAO) using a nylon-monofilament suture remains unsettled, regarding the usefulness of coated or uncoated sutures. Three investigators with different degrees of experience: A, well skilled; B, 2 years of experience; C, a novice with 6 months of experience, each subjected 10 Sprague-Dawley rats to permanent MCAO using low-viscosity silicone-coated sutures with a mean diameter 0.468+/-0.013 mm (mean+/-S.D.) at the tip and 0.361+/-0.013 mm in the body. Post-mortem corrected infarct size 24 h after MCAO was similar among the three investigators: A, 204.7+/-33.2 mm3; B, 212.6+/-42.8, and C, 195.9+/-44.4. The coefficient of variation was 16.2% to 22.7%, and 19.4% for the three investigators. This study suggests that experimental stroke with silicone-coated sutures (Koizumi's method) provides good reproducibility and reliability, among investigators of varying experience.


J Neurol Sci. 1997 Dec 9;153(1):8-11.

Journal/Book/Conference Title

Journal of the neurological sciences

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