Title
Reproducibility and reliability of middle cerebral artery occlusion using a silicone-coated suture (Koizumi) in rats
UMMS Affiliation
Department of Neurology
Publication Date
1997-12-09
Document Type
Article
Subjects
Animals; Brain Ischemia; Cerebral Arteries; Disease Models, Animal; Male; Rats; Rats, Sprague-Dawley; Silicones; *Sutures
Disciplines
Nervous System Diseases | Neurology
Abstract
The best technical approach to rat middle cerebral artery occlusion (MCAO) using a nylon-monofilament suture remains unsettled, regarding the usefulness of coated or uncoated sutures. Three investigators with different degrees of experience: A, well skilled; B, 2 years of experience; C, a novice with 6 months of experience, each subjected 10 Sprague-Dawley rats to permanent MCAO using low-viscosity silicone-coated sutures with a mean diameter 0.468+/-0.013 mm (mean+/-S.D.) at the tip and 0.361+/-0.013 mm in the body. Post-mortem corrected infarct size 24 h after MCAO was similar among the three investigators: A, 204.7+/-33.2 mm3; B, 212.6+/-42.8, and C, 195.9+/-44.4. The coefficient of variation was 16.2% to 22.7%, and 19.4% for the three investigators. This study suggests that experimental stroke with silicone-coated sutures (Koizumi's method) provides good reproducibility and reliability, among investigators of varying experience.
Source
J Neurol Sci. 1997 Dec 9;153(1):8-11.
Journal/Book/Conference Title
Journal of the neurological sciences
Related Resources
PubMed ID
9455971
Repository Citation
Takano K, Tatlisumak T, Bergmann AG, Gibson DG, Fisher M. (1997). Reproducibility and reliability of middle cerebral artery occlusion using a silicone-coated suture (Koizumi) in rats. Neurology Publications. Retrieved from https://escholarship.umassmed.edu/neuro_pp/106