Title
Identification and analysis of essential genes in Haemophilus influenzae
UMMS Affiliation
Department of Molecular Genetics and Microbiology
Publication Date
2008-04-09
Document Type
Article
Subjects
DNA Transposable Elements; DNA, Bacterial; DNA-Binding Proteins; *Genes, Bacterial; *Genes, Essential; *Genome, Bacterial; Haemophilus influenzae; Mutagenesis, Insertional; Transposases
Disciplines
Microbiology | Molecular Genetics
Abstract
The human respiratory pathogen Haemophilus influenzae, a Gram-negative bacterium, is the first free-living organism to have its complete genome sequenced, providing the opportunity to apply genomic-scale approaches to study gene function. This chapter provides an overview of a highly efficient, in vitro mariner transposon-based method that exploits the natural transformation feature of this organism for the identification of essential genes. In addition, we describe strategies for conditional expression systems that would facilitate further analysis of this class of genes. Finally, we outline a method based on the approach used in H. influenzae for identifying essential genes that can be applied to other bacteria that are not naturally transformable.
DOI of Published Version
10.1007/978-1-59745-321-9_3
Source
Methods Mol Biol. 2008;416:27-44. Link to article on publisher's site
Journal/Book/Conference Title
Methods in molecular biology (Clifton, N.J.)
Related Resources
Repository Citation
Wong, Sandy M. S. and Akerley, Brian J., "Identification and analysis of essential genes in Haemophilus influenzae" (2008). Molecular Genetics and Microbiology Publications and Presentations. 26.
https://escholarship.umassmed.edu/mgm_pp/26