A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae
Authors
Akerley, Brian J.Rubin, Eric J.
Novik, Veronica N.
Amaya, Kensey
Judson, Nicholas
Mekalanos, John J.
UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyDocument Type
Journal ArticlePublication Date
2002-01-24Keywords
Base SequenceChromosome Mapping
Chromosomes, Bacterial
Genes, Bacterial
*Genome, Bacterial
Haemophilus influenzae
Mutagenesis, Insertional
Open Reading Frames
Phenotype
Plasmids
Microbiology
Molecular Genetics
Metadata
Show full item recordAbstract
A high-density transposon mutagenesis strategy was applied to the Haemophilus influenzae genome to identify genes required for growth or viability. This analysis detected putative essential roles for the products of 259 ORFs of unknown function. Comparisons between complete genomes defined a subset of these proteins in H. influenzae having homologs in Mycobacterium tuberculosis that are absent in Saccharomyces cerevisiae, a distribution pattern that favors their use in development of antimicrobial therapeutics. Three genes within this set are essential for viability in other bacteria. Interfacing the set of essential gene products in H. influenzae with the distribution of homologs in other microorganisms can detect components of unrecognized cellular pathways essential in diverse bacteria. This genome-scale phenotypic analysis identifies potential roles for a large set of genes of unknown function.Source
Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):966-71. Link to article on publisher's siteDOI
10.1073/pnas.012602299Permanent Link to this Item
http://hdl.handle.net/20.500.14038/37351Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1073/pnas.012602299