UMMS Affiliation

Department of Cell and Developmental Biology; UMass Metabolic Network; Imbalzano Lab

Publication Date


Document Type



Biochemistry | Cell Biology | Cellular and Molecular Physiology | Molecular Biology


The eukaryotic genome is partitioned into topologically associating domains (TADs). Despite recent advances characterizing TADs and TAD boundaries, the organization of these structures is an important dimension of genome architecture and function that is not well understood. Recently, we demonstrated that knockdown of BRG1, an ATPase driving the chromatin remodeling activity of mammalian SWI/SNF enzymes, globally alters long-range genomic interactions and results in a reduction of TAD boundary strength. We provided evidence suggesting that this effect may be due to BRG1 affecting nucleosome occupancy around CTCF sites present at TAD boundaries. In this review, we elaborate on our findings and speculate that BRG1 may contribute to the regulation of the structural and functional properties of chromatin at TAD boundaries by affecting the function or the recruitment of CTCF and DNA topoisomerase complexes.


BRG1, CTCF, Hi-C, SWI/SNF, TADs, topoisomerase, topologically associated domains

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Copyright © 2017 The Author(s).

DOI of Published Version



Nucleus. 2017 Mar 4;8(2):150-155. Epub 2017 Jan 6. Link to article on publisher's site

Journal/Book/Conference Title

Nucleus (Austin, Tex.)

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Link to Article in PubMed

PubMed ID


Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.