Department of Cell and Developmental Biology; UMass Metabolic Network; Imbalzano Lab
Biochemistry | Cell Biology | Cellular and Molecular Physiology | Molecular Biology
The eukaryotic genome is partitioned into topologically associating domains (TADs). Despite recent advances characterizing TADs and TAD boundaries, the organization of these structures is an important dimension of genome architecture and function that is not well understood. Recently, we demonstrated that knockdown of BRG1, an ATPase driving the chromatin remodeling activity of mammalian SWI/SNF enzymes, globally alters long-range genomic interactions and results in a reduction of TAD boundary strength. We provided evidence suggesting that this effect may be due to BRG1 affecting nucleosome occupancy around CTCF sites present at TAD boundaries. In this review, we elaborate on our findings and speculate that BRG1 may contribute to the regulation of the structural and functional properties of chromatin at TAD boundaries by affecting the function or the recruitment of CTCF and DNA topoisomerase complexes.
BRG1, CTCF, Hi-C, SWI/SNF, TADs, topoisomerase, topologically associated domains
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Copyright © 2017 The Author(s).
DOI of Published Version
Nucleus. 2017 Mar 4;8(2):150-155. Epub 2017 Jan 6. Link to article on publisher's site
Nucleus (Austin, Tex.)
Barutcu AR, Lian JB, Stein JL, Stein GS, Imbalzano AN. (2017). The connection between BRG1, CTCF and topoisomerases at TAD boundaries. UMass Metabolic Network Publications. https://doi.org/10.1080/19491034.2016.1276145. Retrieved from https://escholarship.umassmed.edu/metnet_pubs/85
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