UMMS Affiliation
Department of Microbiology and Physiological Systems; Department of Biochemistry and Molecular Pharmacology; Proteomics and Mass Spectrometry Facility; UMass Metabolic Network
Publication Date
2016-05-10
Document Type
Article
Disciplines
Biochemistry | Cell Biology | Cellular and Molecular Physiology | Microbiology | Molecular Biology
Abstract
Protected from host immune attack and antibiotic penetration by their unique cell envelope, mycobacterial pathogens cause devastating human diseases such as tuberculosis. Seamless coordination of cell growth with cell envelope elongation at the pole maintains this barrier. Unraveling this spatiotemporal regulation is a potential strategy for controlling mycobacterial infections. Our biochemical analysis previously revealed two functionally distinct membrane fractions in Mycobacterium smegmatis cell lysates: plasma membrane tightly associated with the cell wall (PM-CW) and a distinct fraction of pure membrane free of cell wall components (PMf). To provide further insight into the functions of these membrane fractions, we took the approach of comparative proteomics and identified more than 300 proteins specifically associated with the PMf, including essential enzymes involved in cell envelope synthesis such as a mannosyltransferase, Ppm1, and a galactosyltransferase, GlfT2. Furthermore, comparative lipidomics revealed the distinct lipid composition of the PMf, with specific association of key cell envelope biosynthetic precursors. Live-imaging fluorescence microscopy visualized the PMf as patches of membrane spatially distinct from the PM-CW and notably enriched in the pole of the growing cells. Taken together, our study provides the basis for assigning the PMf as a spatiotemporally distinct and metabolically active membrane domain involved in cell envelope biogenesis.
Keywords
cell envelope, lipid biosynthesis, membrane domain, mycobacteria, polar growth
Rights and Permissions
Publisher PDF posted as allowed by the publisher's author rights policy at http://www.pnas.org/site/aboutpnas/authorfaq.xhtml.
DOI of Published Version
10.1073/pnas.1525165113
Source
Proc Natl Acad Sci U S A. 2016 May 10;113(19):5400-5. 10.1073/pnas.1525165113. Epub 2016 Apr 25. Link to article on publisher's site
Journal/Book/Conference Title
Proceedings of the National Academy of Sciences of the United States of America
Related Resources
PubMed ID
27114527
Repository Citation
Hayashi, Jennifer M.; Leszyk, John D.; Baer, Christina E.; Shaffer, Scott A.; Sassetti, Christopher M.; and Morita, Yasu S., "Spatially distinct and metabolically active membrane domain in mycobacteria" (2016). UMass Metabolic Network Publications. 43.
https://escholarship.umassmed.edu/metnet_pubs/43
Included in
Biochemistry Commons, Cell Biology Commons, Cellular and Molecular Physiology Commons, Microbiology Commons, Molecular Biology Commons
Comments
Full author list omitted for brevity. For the full list of authors, see article.