UMMS Affiliation
Department of Microbiology and Physiological Systems; UMass Metabolic Network
Publication Date
2016-05-27
Document Type
Article
Disciplines
Biochemistry | Cellular and Molecular Physiology | Microbiology | Molecular Biology
Abstract
Little is known about iron efflux transporters within bacterial systems. Recently, the participation of Bacillus subtilis PfeT, a P1B4-ATPase, in cytoplasmic Fe(2+) efflux has been proposed. We report here the distinct roles of mycobacterial P1B4-ATPases in the homeostasis of Co(2+) and Fe(2+) Mutation of Mycobacterium smegmatis ctpJ affects the homeostasis of both ions. Alternatively, an M. tuberculosis ctpJ mutant is more sensitive to Co(2+) than Fe(2+), whereas mutation of the homologous M. tuberculosis ctpD leads to Fe(2+) sensitivity but no alterations in Co(2+) homeostasis. In vitro, the three enzymes are activated by both Fe(2+) and Co(2+) and bind 1 eq of either ion at their transport site. However, equilibrium binding affinities and activity kinetics show that M. tuberculosis CtpD has higher affinity for Fe(2+) and twice the Fe(2+)-stimulated activity than the CtpJs. These parameters are paralleled by a lower activation and affinity for Co(2+) Analysis of Fe(2+) and Co(2+) binding to CtpD by x-ray absorption spectroscopy shows that both ions are five- to six-coordinate, constrained within oxygen/nitrogen environments with similar geometries. Mutagenesis studies suggest the involvement of invariant Ser, His, and Glu residues in metal coordination. Interestingly, replacement of the conserved Cys at the metal binding pocket leads to a large reduction in Fe(2+) but not Co(2+) binding affinity. We propose that CtpJ ATPases participate in the control of steady state Fe(2+) levels. CtpD, required for M. tuberculosis virulence, is a high affinity Fe(2+) transporter involved in the rapid response to iron dyshomeostasis generated upon redox stress.
Keywords
ATPase, Mycobacterium tuberculosis, P1B4-ATPase, iron, metal homeostasis, metal ion-protein interaction, transport metal
Rights and Permissions
Publisher PDF posted after 12 months as allowed by the publisher's author rights policy at http://www.jbc.org/site/misc/Copyright_Permission.xhtml.
DOI of Published Version
10.1074/jbc.M116.718239
Source
J Biol Chem. 2016 May 27;291(22):11529-39. doi: 10.1074/jbc.M116.718239. Epub 2016 Mar 28. Link to article on publisher's site
Journal/Book/Conference Title
The Journal of biological chemistry
Related Resources
PubMed ID
27022029
Repository Citation
Patel, Sarju J.; Lewis, Brianne E.; Long, Jarukit E.; Nambi, Subhalaxmi; Sassetti, Christopher M.; Stemmler, Timothy L.; and Arguello, Jose M., "Fine-tuning of Substrate Affinity Leads to Alternative Roles of Mycobacterium tuberculosis Fe2+-ATPases" (2016). UMass Metabolic Network Publications. 42.
https://escholarship.umassmed.edu/metnet_pubs/42
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Biochemistry Commons, Cellular and Molecular Physiology Commons, Microbiology Commons, Molecular Biology Commons