Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome
Department of Biochemistry and Molecular Pharmacology; UMass Metabolic Network
Biochemistry | Cell Biology | Cellular and Molecular Physiology | Genomics | Molecular Biology
We present Micro-C XL, an improved method for analysis of chromosome folding at mononucleosome resolution. Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome.
DOI of Published Version
Nat Methods. 2016 Dec;13(12):1009-1011. doi: 10.1038/nmeth.4025. Epub 2016 Oct 10. Link to article on publisher's site
Hsieh TS, Fudenberg G, Goloborodko A, Rando OJ. (2016). Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome. UMass Metabolic Network Publications. https://doi.org/10.1038/nmeth.4025. Retrieved from https://escholarship.umassmed.edu/metnet_pubs/21