UMMS Affiliation
Department of Medicine; UMass Metabolic Network
Publication Date
2016-10-17
Document Type
Article
Disciplines
Biochemistry | Cell Biology | Cellular and Molecular Physiology | Molecular Biology
Abstract
It is well established that Ly6Chi monocytes develop from common monocyte progenitors (cMoPs) and reside in the bone marrow (BM) until they are mobilized into the circulation. In our study, we found that BM Ly6Chi monocytes are not a homogenous population, as current data would suggest. Using computational analysis approaches to interpret multidimensional datasets, we demonstrate that BM Ly6Chi monocytes consist of two distinct subpopulations (CXCR4hi and CXCR4lo subpopulations) in both mice and humans. Transcriptome studies and in vivo assays revealed functional differences between the two subpopulations. Notably, the CXCR4hi subset proliferates and is immobilized in the BM for the replenishment of functionally mature CXCR4lo monocytes. We propose that the CXCR4hi subset represents a transitional premonocyte population, and that this sequential step of maturation from cMoPs serves to maintain a stable pool of BM monocytes. Additionally, reduced CXCR4 expression on monocytes, upon their exit into the circulation, does not reflect its diminished role in monocyte biology. Specifically, CXCR4 regulates monocyte peripheral cellular activities by governing their circadian oscillations and pulmonary margination, which contributes toward lung injury and sepsis mortality. Together, our study demonstrates the multifaceted role of CXCR4 in defining BM monocyte heterogeneity and in regulating their function in peripheral tissues.
Keywords
monocytes, CXCR4, premonocytes, bone marrow
Rights and Permissions
© 2016 Chong et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
DOI of Published Version
10.1084/jem.20160800
Source
J Exp Med. 2016 Oct 17;213(11):2293-2314. Epub 2016 Oct 10. Link to article on publisher's site
Journal/Book/Conference Title
The Journal of experimental medicine
Related Resources
PubMed ID
27811056
Repository Citation
Chong, Shu Zhen; Harris, John E.; and Ng, Lai Guan, "CXCR4 Identifies Transitional Bone Marrow Premonocytes that Replenish the Mature Monocyte Pool for Peripheral Responses" (2016). UMass Metabolic Network Publications. 134.
https://escholarship.umassmed.edu/metnet_pubs/134
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.
Included in
Biochemistry Commons, Cell Biology Commons, Cellular and Molecular Physiology Commons, Molecular Biology Commons
Comments
Full list of authors omitted for brevity. For full list see article.