UMMS Affiliation

Department of Molecular, Cell and Cancer Biology

Publication Date

2015-12-01

Document Type

Letter to the Editor

Disciplines

Cancer Biology | Cell Biology | Cellular and Molecular Physiology | Genetics | Genomics | Hematology | Molecular Biology | Molecular Genetics | Oncology

Abstract

We have now examined the erythroid phenotype in this zebrafish strain carrying a ZFN genomic knockout of piezo1. Genotyping was performed as previously described. In contrast to the anemic phenotype observed in zebrafish subjected to morpholino knockdown of piezo, the genomic ZFN knockout of piezo1 did not segregate either with anemia in the 3-dpf embryo or with dysmorphic erythrocyte morphology in the adult fish.

Keywords

PIEZO1, anemia, erythrocyte, morpholino oligomer, zebrafish, zinc-finger nuclease

Rights and Permissions

Haematologica is an Open Access Publication according to the Budapest Open Access Initiative (per http://www.haematologica.org/about-the-journal).

DOI of Published Version

10.3324/haematol.2015.132449

Source

Haematologica. 2015 Dec;100(12):e483-5. doi: 10.3324/haematol.2015.132449. Epub 2015 Aug 20. Link to article on publisher's site

Journal/Book/Conference Title

Haematologica

Related Resources

Link to Article in PubMed

PubMed ID

26294733

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

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