UMMS Affiliation
Department of Molecular, Cell and Cancer Biology
Publication Date
12-1-2015
Document Type
Letter to the Editor
Disciplines
Cancer Biology | Cell Biology | Cellular and Molecular Physiology | Genetics | Genomics | Hematology | Molecular Biology | Molecular Genetics | Oncology
Abstract
We have now examined the erythroid phenotype in this zebrafish strain carrying a ZFN genomic knockout of piezo1. Genotyping was performed as previously described. In contrast to the anemic phenotype observed in zebrafish subjected to morpholino knockdown of piezo, the genomic ZFN knockout of piezo1 did not segregate either with anemia in the 3-dpf embryo or with dysmorphic erythrocyte morphology in the adult fish.
Keywords
PIEZO1, anemia, erythrocyte, morpholino oligomer, zebrafish, zinc-finger nuclease
Rights and Permissions
Haematologica is an Open Access Publication according to the Budapest Open Access Initiative (per http://www.haematologica.org/about-the-journal).
DOI of Published Version
10.3324/haematol.2015.132449
Source
Haematologica. 2015 Dec;100(12):e483-5. doi: 10.3324/haematol.2015.132449. Epub 2015 Aug 20. Link to article on publisher's site
Journal/Book/Conference Title
Haematologica
Related Resources
PubMed ID
26294733
Repository Citation
Shmukler, Boris E.; Huston, Nicholas C.; Thon, Jonathan N.; Ni, Chih-Wen; Kourkoulis, George; Lawson, Nathan D.; Paw, Barry H.; and Alper, Seth L., "Homozygous knockout of the piezo1 gene in the zebrafish is not associated with anemia" (2015). Molecular, Cell and Cancer Biology Publications. 45.
https://escholarship.umassmed.edu/mccb_pubs/45
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
Included in
Cancer Biology Commons, Cell Biology Commons, Cellular and Molecular Physiology Commons, Genetics Commons, Genomics Commons, Hematology Commons, Molecular Biology Commons, Molecular Genetics Commons, Oncology Commons