UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; Program in Cell and Developmental Dynamics; Department of Molecular, Cell and Cancer Biology
Publication Date
2015-03-10
Document Type
Article
Disciplines
Biochemistry | Cell Biology | Cells | Molecular Biology
Abstract
The intranuclear location of genomic loci and the dynamics of these loci are important parameters for understanding the spatial and temporal regulation of gene expression. Recently it has proven possible to visualize endogenous genomic loci in live cells by the use of transcription activator-like effectors (TALEs), as well as modified versions of the bacterial immunity clustered regularly interspersed short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Here we report the design of multicolor versions of CRISPR using catalytically inactive Cas9 endonuclease (dCas9) from three bacterial orthologs. Each pair of dCas9-fluorescent proteins and cognate single-guide RNAs (sgRNAs) efficiently labeled several target loci in live human cells. Using pairs of differently colored dCas9-sgRNAs, it was possible to determine the intranuclear distance between loci on different chromosomes. In addition, the fluorescence spatial resolution between two loci on the same chromosome could be determined and related to the linear distance between them on the chromosome's physical map, thereby permitting assessment of the DNA compaction of such regions in a live cell.
Keywords
4D nucleome, telomeres, pericentromeric DNA, chromosomes
Rights and Permissions
Freely available online through the PNAS open access option.
DOI of Published Version
10.1073/pnas.1420024112
Source
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Link to article on publisher's site
Journal/Book/Conference Title
Proceedings of the National Academy of Sciences of the United States of America
Related Resources
PubMed ID
25713381
Repository Citation
Ma, Hanhui; Naseri, Ardalan; Reyes-Gutierrez, Pablo; Wolfe, Scot A.; Zhang, Shaojie; and Pederson, Thoru, "Multicolor CRISPR labeling of chromosomal loci in human cells" (2015). Molecular, Cell and Cancer Biology Publications. 10.
https://escholarship.umassmed.edu/mccb_pubs/10
Comments
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1420024112/-/DCSupplemental.