Title

Cloning the Bacillus subtilis 168 aroC gene encoding dehydroquinase

UMMS Affiliation

Department of Microbiology; Department of Microbiology and Physiological Systems

Publication Date

1984-12-01

Document Type

Article

Disciplines

Genetics and Genomics | Microbiology | Physiology

Abstract

An approx. 14-kb Sau3A fragment of Bacillus subtilis DNA containing the aroC and ser-22 genes has been isolated. Gene aroC is expressed in both B. subtilis and Escherichia coli and appears to contain its own promoter, allowing complementation in B. subtilis. However, expression in E. coli is dependent on insert orientation, so the direction of transcription can be deduced. The level of dehydroquinase-specific activity, encoded by the cloned aroC gene, is raised 30- to 40-fold in both E. coli and B. subtilis. The clones are stable in both E. coli and B. subtilis but appear to have undergone several large deletions during their construction.

DOI of Published Version

10.1016/0378-1119(84)90032-5

Source

Gene. 1984 Dec;32(1-2):57-66. doi: 10.1016/0378-1119(84)90032-5. Link to article on publisher's site

Journal/Book/Conference Title

Gene

Related Resources

Link to Article in PubMed

PubMed ID

6442253

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