Cloning the Bacillus subtilis 168 aroC gene encoding dehydroquinase
Department of Microbiology; Department of Microbiology and Physiological Systems
Genetics and Genomics | Microbiology | Physiology
An approx. 14-kb Sau3A fragment of Bacillus subtilis DNA containing the aroC and ser-22 genes has been isolated. Gene aroC is expressed in both B. subtilis and Escherichia coli and appears to contain its own promoter, allowing complementation in B. subtilis. However, expression in E. coli is dependent on insert orientation, so the direction of transcription can be deduced. The level of dehydroquinase-specific activity, encoded by the cloned aroC gene, is raised 30- to 40-fold in both E. coli and B. subtilis. The clones are stable in both E. coli and B. subtilis but appear to have undergone several large deletions during their construction.
DOI of Published Version
Gene. 1984 Dec;32(1-2):57-66. doi: 10.1016/0378-1119(84)90032-5. Link to article on publisher's site
Warburg RJ, Mahler I, Tipper DJ, Halvorson HO. (1984). Cloning the Bacillus subtilis 168 aroC gene encoding dehydroquinase. Microbiology and Physiological Systems Publications. https://doi.org/10.1016/0378-1119(84)90032-5. Retrieved from https://escholarship.umassmed.edu/maps_pubs/65