UMMS Affiliation

Department of Microbiology; Department of Microbiology and Physiological Systems

Publication Date


Document Type



Bacteriology | Microbiology | Physiology


In synchronously sporulating cells of Bacillus sphaericus 9602, the specific activities of those enzymes specifically required for the synthesis of the UDP-N-acetyl-muramyl-pentapeptide precursor of vegetative cell wall peptidoglycan decay by 50% after the end of exponential cell division, probably as a consequence of dilution by newly synthesized protein. The meso-diaminopimelate ligase is the only new activity whose synthesis is required for synthesis of the nucleotide-pentapeptide precursor of spore cortex peptidoglycan. The addition of d-Ala-d-Ala to the nucleotide tripeptide is catalyzed by an enzyme present in both vegetative and sporulating cells, which apparently does not discriminate between lysine- and diaminopimelate-containing acceptors. The activities of the l-Ala and d-Ala-d-Ala ligases and of the d-Ala-d-Ala synthetase increases in parallel with the appearance of the diaminopimelate ligase, indicating coordinate derepression and suggesting operon-like organization of the appropriate structural genes.

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Copyright © 1974, American Society for Microbiology. Publisher PDF posted as allowed by the publisher's copyright policy at


J Bacteriol. 1974 Oct;120(1):342-54. Link to article on publisher's site

Journal/Book/Conference Title

Journal of bacteriology

Related Resources

Link to Article in PubMed

PubMed ID