UMMS Affiliation

Department of Molecular Genetics and Microbiology; Department of Microbiology and Physiological Systems

Publication Date

1983-02-25

Document Type

Article

Disciplines

Biochemistry | Microbiology | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides

Abstract

In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and partial-length (la,ma) single-stranded species. Both L-dsRNA transcripts (l,la) have in vitro mRNA activity for L-P1, previously shown to be identical to ScV-P1, the 88,000 dalton major capsid protein of the virus-like particles containing L- and M1-dsRNAs. 1, but not 1a, is bound to poly(U)-sepharose and may be polyadenylated. Other L-dsRNA gene products and their transcripts may exist. For M1-dsRNA, both species (m, ma) have in vitro mRNA activity for M1-P1, the 32,000 dalton pre-protoxin encoded by M1-dsRNA. Both m and ma are bound to poly(U)-Sepharose and ma is probably a 5' terminal fragment of m. A functional model for M1-dsRNA killer plasmid structure is presented.

Keywords

Saccharomyces cerevisiae, plasmids, dsRNA, yeast killer

Rights and Permissions

Copyright © 1983 IRL Press Limited, Oxford, England. Publisher PDF posted as allowed by the publisher's author self-archiving policy at https://academic.oup.com/journals/pages/access_purchase/rights_and_permissions/self_archiving_policy_c.

DOI of Published Version

10.1093/nar/11.4.1077

Source

Nucleic Acids Res. 1983 Feb 25;11(4):1077-97. doi: 10.1093/nar/11.4.1077. Link to article on publisher's site

Journal/Book/Conference Title

Nucleic acids research

Related Resources

Link to Article in PubMed

PubMed ID

6338480

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