UMMS Affiliation

Department of Molecular Genetics and Microbiology; Department of Microbiology and Physiological Systems

Publication Date


Document Type



Biochemistry | Microbiology | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides


In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and partial-length (la,ma) single-stranded species. Both L-dsRNA transcripts (l,la) have in vitro mRNA activity for L-P1, previously shown to be identical to ScV-P1, the 88,000 dalton major capsid protein of the virus-like particles containing L- and M1-dsRNAs. 1, but not 1a, is bound to poly(U)-sepharose and may be polyadenylated. Other L-dsRNA gene products and their transcripts may exist. For M1-dsRNA, both species (m, ma) have in vitro mRNA activity for M1-P1, the 32,000 dalton pre-protoxin encoded by M1-dsRNA. Both m and ma are bound to poly(U)-Sepharose and ma is probably a 5' terminal fragment of m. A functional model for M1-dsRNA killer plasmid structure is presented.


Saccharomyces cerevisiae, plasmids, dsRNA, yeast killer

Rights and Permissions

Copyright © 1983 IRL Press Limited, Oxford, England. Publisher PDF posted as allowed by the publisher's author self-archiving policy at

DOI of Published Version



Nucleic Acids Res. 1983 Feb 25;11(4):1077-97. doi: 10.1093/nar/11.4.1077. Link to article on publisher's site

Journal/Book/Conference Title

Nucleic acids research

Related Resources

Link to Article in PubMed

PubMed ID




To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.