Department of Molecular Genetics and Microbiology; Department of Microbiology and Physiological Systems
Biochemistry | Microbiology | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides
In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and partial-length (la,ma) single-stranded species. Both L-dsRNA transcripts (l,la) have in vitro mRNA activity for L-P1, previously shown to be identical to ScV-P1, the 88,000 dalton major capsid protein of the virus-like particles containing L- and M1-dsRNAs. 1, but not 1a, is bound to poly(U)-sepharose and may be polyadenylated. Other L-dsRNA gene products and their transcripts may exist. For M1-dsRNA, both species (m, ma) have in vitro mRNA activity for M1-P1, the 32,000 dalton pre-protoxin encoded by M1-dsRNA. Both m and ma are bound to poly(U)-Sepharose and ma is probably a 5' terminal fragment of m. A functional model for M1-dsRNA killer plasmid structure is presented.
Saccharomyces cerevisiae, plasmids, dsRNA, yeast killer
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Copyright © 1983 IRL Press Limited, Oxford, England. Publisher PDF posted as allowed by the publisher's author self-archiving policy at https://academic.oup.com/journals/pages/access_purchase/rights_and_permissions/self_archiving_policy_c.
DOI of Published Version
Nucleic Acids Res. 1983 Feb 25;11(4):1077-97. doi: 10.1093/nar/11.4.1077. Link to article on publisher's site
Nucleic acids research
Bostian KA, Burn VE, Jayachandran S, Tipper DJ. (1983). Yeast killer dsRNA plasmids are transcribed in vivo to produce full and partial-length plus-stranded RNAs. Microbiology and Physiological Systems Publications. https://doi.org/10.1093/nar/11.4.1077. Retrieved from https://escholarship.umassmed.edu/maps_pubs/49