Department of Molecular Genetics and Microbiology; Department of Microbiology and Physiological Systems
Biochemistry | Microbiology | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides
In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and partial-length (la,ma) single-stranded species. Both L-dsRNA transcripts (l,la) have in vitro mRNA activity for L-P1, previously shown to be identical to ScV-P1, the 88,000 dalton major capsid protein of the virus-like particles containing L- and M1-dsRNAs. 1, but not 1a, is bound to poly(U)-sepharose and may be polyadenylated. Other L-dsRNA gene products and their transcripts may exist. For M1-dsRNA, both species (m, ma) have in vitro mRNA activity for M1-P1, the 32,000 dalton pre-protoxin encoded by M1-dsRNA. Both m and ma are bound to poly(U)-Sepharose and ma is probably a 5' terminal fragment of m. A functional model for M1-dsRNA killer plasmid structure is presented.
Saccharomyces cerevisiae, plasmids, dsRNA, yeast killer
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Copyright © 1983 IRL Press Limited, Oxford, England. Publisher PDF posted as allowed by the publisher's author self-archiving policy at https://academic.oup.com/journals/pages/access_purchase/rights_and_permissions/self_archiving_policy_c.
DOI of Published Version
Nucleic Acids Res. 1983 Feb 25;11(4):1077-97. doi: 10.1093/nar/11.4.1077. Link to article on publisher's site
Nucleic acids research
Bostian, Keith A.; Burn, Virginia E.; Jayachandran, S.; and Tipper, Donald J., "Yeast killer dsRNA plasmids are transcribed in vivo to produce full and partial-length plus-stranded RNAs" (1983). Microbiology and Physiological Systems Publications and Presentations. 49.