Title

Kre1p, the plasma membrane receptor for the yeast K1 viral toxin

UMMS Affiliation

Department of Molecular Genetics and Microbiology; Department of Microbiology and Physiological Systems

Publication Date

2002-02-01

Document Type

Article

Disciplines

Microbiology | Physiology

Abstract

Saccharomyces cerevisiae K1 killer strains are infected by the M1 double-stranded RNA virus encoding a secreted protein toxin that kills sensitive cells by disrupting cytoplasmic membrane function. Toxin binding to spheroplasts is mediated by Kre1p, a cell wall protein initially attached to the plasma membrane by its C-terminal GPI anchor. Kre1p binds toxin directly. Both cells and spheroplasts of Deltakre1 mutants are completely toxin resistant; binding to cell walls and spheroplasts is reduced to 10% and < 0.5%, respectively. Expression of K28-Kre1p, an inactive C-terminal fragment of Kre1p retaining its toxin affinity and membrane anchor, fully restored toxin binding and sensitivity to spheroplasts, while intact cells remained resistant. Kre1p is apparently the toxin membrane receptor required for subsequent lethal ion channel formation.

DOI of Published Version

10.1016/S0092-8674(02)00634-7

Source

Cell. 2002 Feb 8;108(3):395-405.

Journal/Book/Conference Title

Cell

Related Resources

Link to Article in PubMed

PubMed ID

11853673

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