Department of Microbiology and Physiological Systems; Division of Infectious Diseases and Immunology, Department of Medicine; Division of Gastroenterology, Department of Medicine
Computational Biology | Genomics | Immunology of Infectious Disease | Pathogenic Microbiology | Virology
The flaviviruses dengue virus (DENV) and Zika virus (ZIKV) are severe health threats with rapidly expanding ranges. To identify the host cell dependencies of DENV and ZIKV, we completed orthologous functional genomic screens using RNAi and CRISPR/Cas9 approaches. The screens recovered the ZIKV entry factor AXL as well as multiple host factors involved in endocytosis (RAB5C and RABGEF), heparin sulfation (NDST1 and EXT1), and transmembrane protein processing and maturation, including the endoplasmic reticulum membrane complex (EMC). We find that both flaviviruses require the EMC for their early stages of infection. Together, these studies generate a high-confidence, systems-wide view of human-flavivirus interactions and provide insights into the role of the EMC in flavivirus replication.
Zika virus, dengue virus, yellow fever virus, flavivirus, arbovirus, EMC, AXL, CRISPR/Cas9 screen, MORR, RNAi screen
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Copyright 2016 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI of Published Version
Savidis et al., Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics, Cell Reports (2016), doi:10.1016/j.celrep.2016.06.028. Link to article on publisher's website
Savidis, George; McDougall, William M.; Meraner, Paul; Perreira, Jill; Portmann, Jocelyn M.; Trincucci, Gaia; John, Sinu P.; Aker, Aaron M.; Renzette, Nicholas; Robbins, Douglas R.; Guo, Zhiru; Green, Sharone; Kowalik, Timothy F.; and Brass, Abraham L., "Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics" (2016). Microbiology and Physiological Systems Publications and Presentations. 16.
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