UMMS Affiliation

Department of Microbiology and Physiological Systems; Division of Infectious Diseases and Immunology, Department of Medicine; Division of Gastroenterology, Department of Medicine

Publication Date

6-21-2016

Document Type

Article

Disciplines

Computational Biology | Genomics | Immunology of Infectious Disease | Pathogenic Microbiology | Virology

Abstract

The flaviviruses dengue virus (DENV) and Zika virus (ZIKV) are severe health threats with rapidly expanding ranges. To identify the host cell dependencies of DENV and ZIKV, we completed orthologous functional genomic screens using RNAi and CRISPR/Cas9 approaches. The screens recovered the ZIKV entry factor AXL as well as multiple host factors involved in endocytosis (RAB5C and RABGEF), heparin sulfation (NDST1 and EXT1), and transmembrane protein processing and maturation, including the endoplasmic reticulum membrane complex (EMC). We find that both flaviviruses require the EMC for their early stages of infection. Together, these studies generate a high-confidence, systems-wide view of human-flavivirus interactions and provide insights into the role of the EMC in flavivirus replication.

Keywords

Zika virus, dengue virus, yellow fever virus, flavivirus, arbovirus, EMC, AXL, CRISPR/Cas9 screen, MORR, RNAi screen

Rights and Permissions

Copyright 2016 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

DOI of Published Version

10.1016/j.celrep.2016.06.028

Source

Savidis et al., Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics, Cell Reports (2016), doi:10.1016/j.celrep.2016.06.028. Link to article on publisher's website

Journal/Book/Conference Title

Cell Reports

Comments

The in press corrected proof is available for download.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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