UMMS Affiliation

Department of Microbiology and Physiological Systems; Division of Infectious Diseases and Immunology, Department of Medicine; Division of Gastroenterology, Department of Medicine

Date

6-21-2016

Document Type

Article

Disciplines

Computational Biology | Genomics | Immunology of Infectious Disease | Pathogenic Microbiology | Virology

Abstract

The flaviviruses dengue virus (DENV) and Zika virus (ZIKV) are severe health threats with rapidly expanding ranges. To identify the host cell dependencies of DENV and ZIKV, we completed orthologous functional genomic screens using RNAi and CRISPR/Cas9 approaches. The screens recovered the ZIKV entry factor AXL as well as multiple host factors involved in endocytosis (RAB5C and RABGEF), heparin sulfation (NDST1 and EXT1), and transmembrane protein processing and maturation, including the endoplasmic reticulum membrane complex (EMC). We find that both flaviviruses require the EMC for their early stages of infection. Together, these studies generate a high-confidence, systems-wide view of human-flavivirus interactions and provide insights into the role of the EMC in flavivirus replication.

Rights and Permissions

Citation: Savidis et al., Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics, Cell Reports (2016), doi:10.1016/j.celrep.2016.06.028. Link to article on publisher's website

Copyright 2016 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Comments

The in press corrected proof is available for download.

Keywords

Zika virus, dengue virus, yellow fever virus, flavivirus, arbovirus, EMC, AXL, CRISPR/Cas9 screen, MORR, RNAi screen

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.