Developmental expression of a candidate mullerian inhibiting substance type II receptor

UMMS Affiliation

Deptartment of Pediatrics

Publication Date


Document Type



Aging; Amino Acid Sequence; Animals; Animals, Newborn; Base Sequence; Blotting, Northern; *Embryonic and Fetal Development; Female; Fetus; In Situ Hybridization; Male; Molecular Probes; Molecular Sequence Data; Rats; Receptors, Peptide; Receptors, Transforming Growth Factor beta


Cell Biology | Developmental Biology | Endocrinology


We have isolated a candidate Mullerian inhibiting substance (MIS) type II receptor complementary DNA from an embryonic rat urogenital ridge library and have studied its binding to MIS, its developmental pattern of expression and tissue distribution. By in situ hybridization with a full-length riboprobe, the receptor is expressed in the mesenchymal cells surrounding the Mullerian duct at embryonic days 14, 15, and 16 and in tubular and follicular structures of the rat fetal gonads. Expression of the messenger RNA was also seen in the granules cells and seminiferous tubules of pubertal gonads. Northern analysis revealed that the MIS type II receptor messenger RNA is highly expressed in embryonic, pubertal, and adult testes and ovaries, as well as in the gravid uterus. The timing of expression in the gonads of both sexes was also analyzed by Northern analyses that showed high levels of expression at the time of Mullerian duct regression, much lower levels neonatally and prepubertally and then increased expression again with sexual maturation. The tissue and developmental specificity of expression of this receptor, which make it likely that this is the functional MIS type II receptor, can be used to advantage in therapeutic targeting strategies and to decipher the function of MIS in the gonads.

DOI of Published Version



Endocrinology. 1996 Jan;137(1):160-5. Link to article on publisher's site

Journal/Book/Conference Title



At the time of publication, Mary Lee was not yet affiliated with the University of Massachusetts Medical School.

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Link to Article in PubMed

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