Developmental changes in mullerian inhibiting substance in the cynomolgus monkey, Macaca fascicularis

UMMS Affiliation

Department of Pediatrics

Publication Date


Document Type



Aging; Animals; Anti-Mullerian Hormone; Blotting, Northern; Enzyme-Linked Immunosorbent Assay; Gene Expression Regulation; *Glycoproteins; Growth Inhibitors; In Situ Hybridization; Macaca fascicularis; Mullerian Ducts; Osmolar Concentration; RNA, Messenger; Testicular Hormones


Cell Biology | Developmental Biology | Endocrinology


Mullerian inhibiting substance (MIS) is a glycoprotein hormone produced in Sertoli cells of the fetal and postnatal testis, and granulosa cells of the pubertal ovary. We examined MIS expression in a nonhuman primate, the cynomolgus macaque monkey (Macaca fascicularis), to define an animal model for studying MIS gene regulation. Changes in testicular MIS mRNA with age were assessed by in situ hybridization of prepubertal to adult testes, Northern analysis of pubertal and adult specimens, and determination of serum MIS concentrations from infancy to adulthood. We found that MIS expression was highest in the youngest animals and decreased progressively with increasing age. Serum MIS concentrations correlated inversely with increasing age (r = -0.74), body weight (r = -0.79), and testicular volume (r = -0.73), but not with testosterone levels (r = -0.35). The mean MIS concentrations +/- SEM for the four developmental age groups were 270.6 +/- 23.8 (infants), 195.5 +/- 18.5 (juveniles), 102.7 +/- 28.4 (peripubertals), and 51.6 +/- 7.1 (adults). This study confirms that nonhuman primate and human MIS are highly homologous and have similar developmental patterns. The normative data for serum MIS concentrations in cynomolgus monkeys at different ages and developmental stages will be invaluable for further work examining MIS regulation.

DOI of Published Version



J Clin Endocrinol Metab. 1994 Mar;78(3):615-21. Link to article on publisher's site

Journal/Book/Conference Title

The Journal of clinical endocrinology and metabolism


At the time of publication, Mary Lee was not yet affiliated with the University of Massachusetts Medical School.

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Link to Article in PubMed

PubMed ID