Dicer is required for the formation of white but not brown adipose tissue
Authors
Mudhasani, Rajini R.Puri, Vishwajeet
Hoover, Kathleen
Czech, Michael P.
Imbalzano, Anthony N.
Jones, Stephen N.
Document Type
Journal ArticlePublication Date
2011-05-15
Metadata
Show full item recordAbstract
Dicer, an enzyme involved in microRNA maturation, is required for proper embryo gastrulation and tissue morphogenesis during mammalian development. Using primary cultures of fibroblasts and pre-adipocytes, we have previously shown that Dicer is essential for early stages of adipogenic cell differentiation. In this present study, we have utilized Dicer-conditional mice to explore a role for Dicer and microRNA biogenesis in the terminal differentiation of adipocytes in vivo and in the formation of white and brown adipose tissue. Deletion of Dicer in differentiated adipocytes in Dicer-conditional, aP2-Cre transgenic mice reduced the level of various adipogenic-associated transcripts and inhibited lipogenesis in white adipocytes, resulting in a severe depletion of white adipose tissue in mice. In contrast, Dicer was not required in vivo for lipogenesis in brown adipose or for brown fat formation. However, Dicer deletion in brown adipose did decrease the expression of genes involved in thermoregulation. The results of our study provide genetic evidence of a role for microRNA molecules in regulating adipogenesis and reveal distinct requirements for Dicer in the formation of white and brown adipose tissue. (c) 2010 Wiley-Liss, Inc.Source
J Cell Physiol. 2011 May;226(5):1399-406. doi: 10.1002/jcp.22475. Link to article on publisher's siteDOI
10.1002/jcp.22475Permanent Link to this Item
http://hdl.handle.net/20.500.14038/36029Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/jcp.22475