Role of substance P in several models of bladder inflammation

UMMS Affiliation

Information Services, Academic Computing Services; Department of Cell Biology; Department of Physiology

Publication Date


Document Type



Animals; Biphenyl Compounds; Cystitis; Disease Models, Animal; Female; Immunohistochemistry; Lipopolysaccharides; Poly I-C; Rats; Receptors, Neurokinin-1; Substance P; Urinary Bladder; Urination; Xylenes


Immunology and Infectious Disease


Substance P (SP) is a peptide found in the sensory nervous system which has multiple biologic effects including stimulation of muscle contraction, pain nociception, immune cell functions, plasma extravasation and a constellation of inflammatory effects. Here we investigate the role of SP in several animals models of bladder inflammation. Using the female Lewis rat, inflammation was induced using either xylene, lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (polyIC). Inflammation occurred rapidly (4 h) and was maintained in each model for at least 7 days. Each of these protocols decreased the bladder content of immunoreactive SP by approximately 50%, suggesting enhanced release. There was no change in the urinary frequency of these animals over 3 weeks, suggesting that urinary frequency changes are not mediated by acute inflammation. We also found that the SP receptor (NK1) antagonist, (-)CP96345, was unable to block the inflammation produced by polyIC, suggesting that SP is not an obligatory mediator of immune cell stimulation in this model.


Urol Res. 1997;25(6):395-9.

Journal/Book/Conference Title

Urological research

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Link to article in PubMed

PubMed ID