Peptidoglycan recognition by the Drosophila Imd pathway

UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology; Department of Molecular Genetics and Microbiology

Publication Date


Document Type



Animals; Bacterial Proteins; Drosophila; Drosophila Proteins; Genes, Reporter; Luciferases; Peptidoglycan; Protein Isoforms; Signal Transduction


Immunology and Infectious Disease


The structural requirements for recognition of peptidoglycan (PGN) by PGRP-LC and activation of the Drosophila IMD pathway are not yet clear. In order to examine this question more carefully, the activity of peptidoglycan from different types of bacteria was compared in cell-based and whole animal assays. Drosophila S2* cells, but not adult flies, responded to Lys-type Micrococcus luteus PGN, but with significantly less potency compared to Dap-type Escherichia coli PGN, while intact Lys-type PGN from Staphylococcus aureus was inactive. After treatment with lysostaphin, which digests the cross-bridging peptides, S. aureus PGN weakly stimulated the IMD pathway, similar to M. luteus PGN. Further digestion with mutanolysin, which creates monomeric PGN fragments, abolished the activity of S. aureus PGN. On the other hand, monomeric E. coli PGN, generated by mutanolysin digestion, was still active but required different isoforms of PGRP-LC for recognition. Polymeric PGN required only PGRP-LCx, while monomeric E. coli PGN required both the PGRP-LCa and PGRP-LCx isoforms. These results suggest that the recognition by PGRP-LCx alone requires polymeric PGN, and that polymeric Dap-type PGN is a more potent PGRP-LCx agonist, compared to Lys-type PGN. These results also suggest that the heteromeric PGRP-LCa/LCx receptor complex recognizes monomeric Dap-type, but not Lys-type, PGN.

DOI of Published Version



J Endotoxin Res. 2005;11(6):383-9. Link to article on publisher's site

Journal/Book/Conference Title

Journal of endotoxin research

Related Resources

Link to Article in PubMed

PubMed ID