Department of Medicine, Division of Infectious Diseases and Immunology
Animals; Databases, Genetic; *Gene Expression; Genomics; Humans; Mice; MicroRNAs; Oligonucleotide Array Sequence Analysis; RNA, Messenger
Immunology and Infectious Disease
BACKGROUND: MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression by binding to the messenger RNA (mRNA) of protein coding genes. They control gene expression by either inhibiting translation or inducing mRNA degradation. A number of computational techniques have been developed to identify the targets of miRNAs. In this study we used predicted miRNA-gene interactions to analyse mRNA gene expression microarray data to predict miRNAs associated with particular diseases or conditions.
RESULTS: Here we combine correspondence analysis, between group analysis and co-inertia analysis (CIA) to determine which miRNAs are associated with differences in gene expression levels in microarray data sets. Using a database of miRNA target predictions from TargetScan, TargetScanS, PicTar4way PicTar5way, and miRanda and combining these data with gene expression levels from sets of microarrays, this method produces a ranked list of miRNAs associated with a specified split in samples. We applied this to three different microarray datasets, a papillary thyroid carcinoma dataset, an in-house dataset of lipopolysaccharide treated mouse macrophages, and a multi-tissue dataset. In each case we were able to identified miRNAs of biological importance.
CONCLUSIONS: We describe a technique to integrate gene expression data and miRNA target predictions from multiple sources.
DOI of Published Version
BMC Bioinformatics. 2010 May 18;11:257. Link to article on publisher's site
Madden, Stephen F.; Carpenter, Susan B.; Jeffery, Ian B.; Bjorkbacka, Harry; Fitzgerald, Katherine A.; O'Neill, Luke A. J.; and Higgins, Desmond G., "Detecting microRNA activity from gene expression data" (2010). Infectious Diseases and Immunology Publications and Presentations. 53.