Activation of Peripheral T Follicular Helper Cells During Acute Dengue Virus Infection
Department of Medicine, Division of Infectious Diseases and Immunology
Immunology and Infectious Disease | Infectious Disease | Virology | Virus Diseases
Background: Follicular helper T cells (TFH) are specialized CD4 T cells required for B-cell help and antibody production.
Methods: Given the postulated role of immune activation in dengue disease, we measured the expansion and activation of TFH in the circulation (peripheral TFH [pTFH]) collected from Thai children with laboratory-confirmed acute dengue virus (DENV) infection.
Results: We found significant expansion and activation of pTFH subsets during acute infection with the highest frequencies of activated pTFH (PD1hi pTFH and PD1+CD38+ pTFH) detected during the critical phase of illness. Numbers of activated pTFH were higher in patients with secondary compared with primary infections and in patients with more severe disease. We also found a positive correlation between the frequencies of activated pTFH and the frequencies of plasmablasts.
Conclusions: To our knowledge, this is the first ex vivo analysis of pTFH activation during acute DENV infection. Overall, our study supports the model that pTFH contribute to disease evolution during the critical stage of illness.
acute infection, children, dengue virus, follicular helper T cells, immune response
DOI of Published Version
Haltaufderhyde K, Srikiatkhachorn A, Green S, Macareo L, Park S, Kalayanarooj S, Rothman AL, Mathew A. Activation of Peripheral T Follicular Helper Cells During Acute Dengue Virus Infection. J Infect Dis. 2018 Oct 5;218(10):1675-1685. doi: 10.1093/infdis/jiy360. PMID: 29917084; PMCID: PMC6927865. Link to article on publisher's site
The Journal of infectious diseases
Haltaufderhyde K, Srikiatkhachorn A, Green S, Macareo L, Park S, Kalayanarooj S, Rothman AL, Mathew A. (2018). Activation of Peripheral T Follicular Helper Cells During Acute Dengue Virus Infection. Infectious Diseases and Immunology Publications. https://doi.org/10.1093/infdis/jiy360. Retrieved from https://escholarship.umassmed.edu/infdis_pp/427