Taming the symbiont for coexistence: a host PGRP neutralizes a bacterial symbiont toxin
Department of Medicine, Division of Infectious Diseases and Immunology
Symbiosis; Decapodiformes; Vibrio fischeri; Carrier Proteins; Peptidoglycan; Morphogenesis
Environmental Microbiology and Microbial Ecology | Immunology and Infectious Disease
In horizontally transmitted mutualisms between marine animals and their bacterial partners, the host environment promotes the initial colonization by specific symbionts that it harvests from the surrounding bacterioplankton. Subsequently, the host must develop long-term tolerance to immunogenic bacterial molecules, such as peptidoglycan and lipopolysaccaride derivatives. We describe the characterization of the activity of a host peptidoglycan recognition protein (EsPGRP2) during establishment of the symbiosis between the squid Euprymna scolopes and its luminous bacterial symbiont Vibrio fischeri. Using confocal immunocytochemistry, we localized EsPGRP2 to all epithelial surfaces of the animal, and determined that it is exported in association with mucus shedding. Most notably, EsPGRP2 was released by the crypt epithelia into the extracellular spaces housing the symbionts. This translocation occurred only after the symbionts had triggered host morphogenesis, a process that is induced by exposure to the peptidoglycan monomer tracheal cytotoxin (TCT), a bacterial 'toxin' that is constitutively exported by V. fischeri. Enzymatic analyses demonstrated that, like many described PGRPs, EsPGRP2 has a TCT-degrading amidase activity. The timing of EsPGRP2 export into the crypts provides evidence that the host does not export this protein until after TCT induces morphogenesis, and thereafter EsPGRP2 is constantly present in the crypts ameliorating the effects of V. fischeri TCT.
DOI of Published Version
Environ Microbiol. 2010 Aug;12(8):2190-203. doi: 10.1111/j.1462-2920.2009.02121.x. Epub 2009 Dec 27. Link to article on publisher's site
Troll, Joshua V.; Bent, Eric H.; Paquette, Nicholas Paul; Wier, Andrew M.; Goldman, William E.; Silverman, Neal S.; and McFall-Ngai, Margaret J., "Taming the symbiont for coexistence: a host PGRP neutralizes a bacterial symbiont toxin" (2010). Infectious Diseases and Immunology Publications and Presentations. 38.