Kinetics and specificity at the clonal level of the cytotoxic T lymphocyte response to influenza pneumonia
Center for Infectious Disease and Vaccine Research; Department of Medicine, Division of Infectious Diseases and Immunology
Immunity | Immunology and Infectious Disease | Immunology of Infectious Disease | Infectious Disease | Virology
We have studied the kinetics and specificity of the cytotoxic T lymphocyte (CTL) response to influenza A/PR/8 (H1N1) virus pulmonary infection in the mouse detected using spleen cells from infected mice which were stimulated in bulk and limiting dilution cultures. A hybrid protein designated D-peptide, which contains the terminal 157 amino acids of the HA2 subunit of A/PR/8 virus, was used to stimulate influenza virus subtype-specific secondary CTL in vitro. Infection induced two specificities of precursor CTL, cross-reactive and subtype-specific. The kinetics of the subtype-specific CTL response detected by the D-peptide were similar to the cross-reactive CTL response detected by stimulation with live virus. The majority of the precursor CTL (CTL-p) are able to lyse virus-infected target cells in a cross-reactive fashion. The number of memory subtype-specific and cross-reactive CTL increased by approximately 2.5 logs10 during the first 3 weeks after infection.
DOI of Published Version
Viral Immunol. 1987-1988;1(4):259-66. doi:10.1089/vim.1987.1.259. Link to article on publisher's site
Jaffe, Penny A.; Kuwano, Koichi; Yamada, Akio; Scott, Miller; Young, James F.; and Ennis, Francis A., "Kinetics and specificity at the clonal level of the cytotoxic T lymphocyte response to influenza pneumonia" (1987). Infectious Diseases and Immunology Publications and Presentations. 341.