Genetic isolation of ADA2: a potential transcriptional adaptor required for function of certain acidic activation domains
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Authors
Berger, Shelley L.Pina, Benjamin
Silverman, Neal S.
Marcus, Gregory A.
Agapite, Julie
Regier, Jeffrey L.
Triezenberg, Steven J.
Guarente, Leonard
UMass Chan Affiliations
Department of Medicine, Division of Infectious Diseases and ImmunologyDocument Type
Journal ArticlePublication Date
1992-07-24Keywords
Amino Acid SequenceBase Sequence
DNA Mutational Analysis
*Genes, Fungal
Genome
Molecular Sequence Data
Saccharomyces cerevisiae
Sequence Alignment
*Trans-Activators
Immunology and Infectious Disease
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Show full item recordAbstract
We have devised a genetic strategy to isolate the target of acidic activation domains of transcriptional activators based on toxicity in yeast cells of the chimeric activator, GAL4-VP16. Toxicity required the integrity of both the VP16 acidic activation domain and the GAL4 DNA-binding domain, suggesting that inhibition resulted from trapping of general transcription factors at genomic sites. Mutations that break the interaction between GAL4-VP16 and general factors would alleviate toxicity and identify transcriptional adaptors, if adaptors bridged the interaction between activators and general factors. We thus identified ADA1, ADA2, and ADA3. Mutations in ADA2 reduced the activity of GAL4-VP16 and GCN4 in vivo. ada2 mutant extracts exhibited normal basal transcription, but were defective in responding to GAL4-VP16, GCN4, or the dA:dT activator. Strikingly, the mutant extract responded like wild type to GAL4-HAP4. We conclude that ADA2 potentiates the activity of one class of acidic activation domain but not a second class.Source
Cell. 1992 Jul 24;70(2):251-65. Link to article on publisher's siteDOI
10.1016/0092-8674(92)90100-QPermanent Link to this Item
http://hdl.handle.net/20.500.14038/35078PubMed ID
1638630Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/0092-8674(92)90100-Q