Title

RNA and beta-hemolysin of Group B streptococcus induce IL-1beta by activating NLRP3 inflammasomes in mouse macrophages

UMMS Affiliation

Department of Medicine, Division of Infectious Diseases and Immunology

Publication Date

2014-04-01

Document Type

Article

Disciplines

Bacterial Infections and Mycoses | Biochemistry | Immunity | Immunology and Infectious Disease | Immunology of Infectious Disease

Abstract

The inflammatory cytokine IL-1beta is critical for host responses against many human pathogens. Here, we define Group B streptococcus (GBS)-mediated activation of the Nod-like Receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, beta-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1beta production.

Keywords

Cell signaling, Immunology, Innate immunity, Interleukin, RNA

DOI of Published Version

10.1074/jbc.C114.548982

Source

Gupta R, Ghosh S, Monks B, Deoliveira R, Tzeng T, Kalantari P, Nandy A, Bhattacharjee B, Chan J, Ferreira F, Rathinam V, Sharma S, Lien E, Silverman N, Fitzgerald K, Firon A, Trieu-Cuot P, Henneke P, Golenbock D. RNA and β-hemolysin of Group B streptococcus induce IL-1β by activating NLRP3 inflammasomes in mouse macrophages. J Biol Chem. 2014 Apr 1. doi:10.1074/jbc.C114.548982. Link to article on publisher's site

Journal/Book/Conference Title

The Journal of biological chemistry

Comments

Co-authors Anubhab Nandy and Jennie Chan are doctoral students in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

24692555

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