RNA and beta-hemolysin of Group B streptococcus induce IL-1beta by activating NLRP3 inflammasomes in mouse macrophages
Department of Medicine, Division of Infectious Diseases and Immunology
Bacterial Infections and Mycoses | Biochemistry | Immunity | Immunology and Infectious Disease | Immunology of Infectious Disease
The inflammatory cytokine IL-1beta is critical for host responses against many human pathogens. Here, we define Group B streptococcus (GBS)-mediated activation of the Nod-like Receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, beta-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1beta production.
Cell signaling, Immunology, Innate immunity, Interleukin, RNA
DOI of Published Version
Gupta R, Ghosh S, Monks B, Deoliveira R, Tzeng T, Kalantari P, Nandy A, Bhattacharjee B, Chan J, Ferreira F, Rathinam V, Sharma S, Lien E, Silverman N, Fitzgerald K, Firon A, Trieu-Cuot P, Henneke P, Golenbock D. RNA and β-hemolysin of Group B streptococcus induce IL-1β by activating NLRP3 inflammasomes in mouse macrophages. J Biol Chem. 2014 Apr 1. doi:10.1074/jbc.C114.548982. Link to article on publisher's site
The Journal of biological chemistry
Gupta, Rahul; Ghosh, Shubhendu; Monks, Brian G.; DeOliveira, Rosane B.; Tzeng, TeChen; Kalantari, Parisa; Nandy, Anubhab; Bhattacharjee, Bornali; Chan, Jennie; Ferreira, Fabianno; Rathinam, Vijay A.K.; Sharma, Shrutie; Lien, Egil; Silverman, Neal S.; Fitzgerald, Katherine A.; Firon, Arnaud; Trieu-Cuot, Patrick; Henneke, Philipp; and Golenbock, Douglas T., "RNA and beta-hemolysin of Group B streptococcus induce IL-1beta by activating NLRP3 inflammasomes in mouse macrophages" (2014). Infectious Diseases and Immunology Publications and Presentations. 158.