The tyrosine kinase c-Src enhances RIG-I (retinoic acid-inducible gene I)-elicited antiviral signaling
Department of Medicine, Division of Infectious Diseases and Immunology
Animals; Antiviral Agents; DEAD-box RNA Helicases; *Gene Expression Regulation; Genes, Reporter; Humans; Interferon Regulatory Factor-3; Mice; Models, Biological; RNA, Small Interfering; Sendai virus; Signal Transduction; TNF Receptor-Associated Factor 3; src-Family Kinases
Immunology and Infectious Disease
Antiviral immune responses are initiated through Toll-like receptors (TLRs) and RIG-I (retinoic acid-inducible gene-I)-like RNA helicases that recognize nucleic acids from distinct viruses. In this study, we show that the tyrosine kinase c-Src participates in antiviral responses induced by the cytoplasmic RNA helicase RIG-I. Sendai virus (SV), which is recognized by RIG-I, induced c-Src phosphorylation. Functional impairment of c-Src through chemical inhibition or transient expression of a c-Src kinase-inactive mutant attenuated production of endogenous antiviral proteins after SV infection or after expression of RIG-I or its adapter protein MAVS. Importantly, SV-stimulated synthesis of antiviral proteins was significantly impaired in cells treated with c-Src small interfering RNA and in cells from c-Src-deficient mice. In addition, we found that c-Src interacted with components of the RIG-I pathway: RIG-I, MAVS, and TRAF3 (tumor necrosis factor receptor-associated factor-3). The interaction between c-Src and TRAF3 was found to occur within the RING domain of TRAF3. Taken together, our results suggest that c-Src enhances RIG-I-mediated signaling, acting at the level of TRAF3.
DOI of Published Version
J Biol Chem. 2009 Jul 10;284(28):19122-31. Epub 2009 May 6. Link to article on publisher's site
The Journal of biological chemistry
Johnsen, Ingvild B.; Nguyen, Thuy Thanh; Bergstroem, Bjarte; Fitzgerald, Katherine A.; and Anthonsen, Marit W., "The tyrosine kinase c-Src enhances RIG-I (retinoic acid-inducible gene I)-elicited antiviral signaling" (2009). Infectious Diseases and Immunology Publications and Presentations. 118.