The LPS receptor generates inflammatory signals from the cell surface
Authors
Latz, EickeVisintin, Alberto
Lien, Egil
Fitzgerald, Katherine A.
Espevik, Terje
Golenbock, Douglas T.
UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyDepartment of Medicine, Division of Infectious Diseases and Immunology
Document Type
Journal ArticlePublication Date
2003-12-22Keywords
Antigens, CD14Cell Line
Cell Membrane
Culture Media
Enzyme-Linked Immunosorbent Assay
Golgi Apparatus
Green Fluorescent Proteins
Humans
*Inflammation
Interleukin-8
Kidney
Lipopolysaccharides
Luminescent Proteins
Microscopy, Confocal
Recombinant Fusion Proteins
*Signal Transduction
Immunology and Infectious Disease
Metadata
Show full item recordAbstract
Bacterial lipopolysaccharides (LPSs) are recognized in mammals by a receptor complex composed of CD14, Toll-like receptor 4 (TLR4), and MD-2. The mechanism of TLR4 function remains to be elucidated. We constructed chimeric TLR molecules C-terminally fused to fluorescent proteins and stably expressed these chimeric constructs in cells. Confocal microscopy revealed TLR4 to be expressed on the plasma membrane and the Golgi apparatus. Time-lapse confocal imaging showed rapid recycling of TLR4/CD14/MD-2 complexes between the Golgi and the plasma membrane. Membrane TLR4 engagement by antibody was sufficient to induce signaling and pharmacological disruption of the Golgi did not affect cellular responses to LPS. Thus, LPS signaling commences after LPS recognition by surface-expressed TLR4 independent of LPS trafficking to the Golgi.Source
J Endotoxin Res. 2003;9(6):375-80. Link to article on publisher's siteDOI
10.1177/09680519030090061101Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34871PubMed ID
14733724Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1177/09680519030090061101