UMMS Affiliation

Department of Cell and Developmental Biology

Publication Date

2013-09-01

Document Type

Article

Disciplines

Cell Biology | Cellular and Molecular Physiology | Molecular Biology

Abstract

We recently reported that reducing the levels of BRG1, the catalytic subunit of mammalian SWI/SNF chromatin remodeling enzymes, induces alterations in nuclear shape in a breast epithelial cell line. Immunostaining the BRG1 knockdown cells with nuclear lamina antibodies revealed a significantly increased frequency of grooves, or invaginations, in the nuclei. Disruption of each of the major cytoplasmic filament systems (actin, tubulin and cytokeratins) had no impact on the BRG1-dependent changes in nuclear shape, indicating that the observed changes in nuclear morphology are unlikely to be a result of alterations in the integrity of the nuclear-cytoplamic contacts in the cell. We propose that the BRG1-dependent nuclear shape changes reflect a role for the chromatin remodeling enzyme in maintaining the structural integrity of the nucleus via global regulation of chromatin structure and dynamics within the nucleus.

Keywords

BRG1, SWI/SNF, chromatin remodeling, chromatin, lamina, nuclear shape, breast epithelia

Rights and Permissions

Copyright 2013 Landes Bioscience. This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

DOI of Published Version

10.4161/cib.25153

Source

Imbalzano AN, Imbalzano KM, Nickerson JA. BRG1, a SWI/SNF chromatin remodeling enzyme ATPase, is required for maintenance of nuclear shape and integrity. Commun Integr Biol. 2013 Sep 1;6(5):e25153. doi: 10.4161/cib.25153. Link to article on publisher's site

Journal/Book/Conference Title

Communicative and integrative biology

Related Resources

Link to Article in PubMed

PubMed ID

24228137

Creative Commons License

Creative Commons Attribution-Noncommercial 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 License

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