Differential expression of components of the retinoic acid signaling pathway in the adult mouse olfactory epithelium
Document Type
Journal ArticlePublication Date
2012-11-01Keywords
TretinoinRetinal Dehydrogenase
Olfactory Mucosa
Receptors, Retinoic Acid
Cell and Developmental Biology
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Show full item recordAbstract
Position within a tissue often correlates with cellular phenotype, for example, differential expression of odorant receptors and cell adhesion molecules across the olfactory mucosa (OM). The association between position and phenotype is often paralleled by gradations in the concentration of retinoic acid (RA), caused by differential expression of the RA synthetic enzymes, the retinaldehyde dehydrogenases (RALDH). We show here that RALDH-1, -2, and -3 are enriched in the sustentacular cells, deep fibroblasts of the lamina propria, and the superficial fibroblasts, respectively, of the ventral and lateral OM as compared to the dorsomedial OM. The shift from high to low expression of the RALDHs matches the boundary defined by the differential expression of OCAM/mamFasII. Further, we found that RA-binding proteins are expressed in the epithelium overlying the RALDH-3 expressing fibroblasts of the lamina propria. Both findings suggest that local alterations in RA concentration may be more important than a gradient of RA across the epithelial plane, per se. In addition, RALDH-3 is found in a small population of basal cells in the ventral and lateral epithelium, which expand and contribute to the neuronal lineage following MeBr lesion. Indeed, transduction with a retrovirus expressing a dominant negative form of retinoic acid receptor type alpha blocks the reappearance of mature, olfactory marker protein (OMP) (+) olfactory neurons as compared to empty vector. These results support the notion of a potential role for RA, both in maintaining the spatial organization of the normal olfactory epithelium and in reestablishing the neuronal population during regeneration after injury.Source
J Comp Neurol. 2012 Nov 1;520(16):3707-26. doi: 10.1002/cne.23124. Link to article on publisher's siteDOI
10.1002/cne.23124Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34780Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1002/cne.23124