Massachusetts Supranational Tuberculosis Reference Laboratory; Center for Health Policy and Research
Anti-Bacterial Agents; Bacteria; Cells, Cultured; Erythrocytes; Fungi; HEK293 Cells; HeLa Cells; Herpesvirus 1, Human; Herpesvirus 2, Human; Humans; Microbial Sensitivity Tests; Plasmodium falciparum; RNA; Reproducibility of Results; Species Specificity; Urine
Bacterial Infections and Mycoses | Genetics and Genomics | Immunology and Infectious Disease | Medical Sciences | Public Health | Respiratory Tract Diseases
With rising rates of drug-resistant infections, there is a need for diagnostic methods that rapidly can detect the presence of pathogens and reveal their susceptibility to antibiotics. Here we propose an approach to diagnosing the presence and drug-susceptibility of infectious diseases based on direct detection of RNA from clinical samples. We demonstrate that species-specific RNA signatures can be used to identify a broad spectrum of infectious agents, including bacteria, viruses, yeast, and parasites. Moreover, we show that the behavior of a small set of bacterial transcripts after a brief antibiotic pulse can rapidly differentiate drug-susceptible and -resistant organisms and that these measurements can be made directly from clinical materials. Thus, transcriptional signatures could form the basis of a uniform diagnostic platform applicable across a broad range of infectious agents.
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Publisher PDF posted as allowed by the publisher's author rights policy at http://www.pnas.org/site/misc/authorfaq.shtml.
DOI of Published Version
Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6217-22. doi: 10.1073/pnas.1119540109. Epub 2012 Apr 2. Link to article on publisher's site
Proceedings of the National Academy of Sciences of the United States of America
Barczak, Amy K.; Gomez, James E.; Kaufmann, Benjamin B.; Hinson, Ella R.; Cosimi, Lisa; Borowsky, Mark L.; Onderdonk, Andrew B.; Stanley, Sarah A.; Kaur, Devinder; Bryant, Kevin F.; Knipe, David M.; Sloutsky, Alexander; and Hung, Deborah T., "RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities" (2012). Center for Health Policy and Research (CHPR) Publications. 86.