Massachusetts Supranational Tuberculosis Reference Laboratory; Center for Health Policy and Research
Anti-Bacterial Agents; Bacteria; Cells, Cultured; Erythrocytes; Fungi; HEK293 Cells; HeLa Cells; Herpesvirus 1, Human; Herpesvirus 2, Human; Humans; Microbial Sensitivity Tests; Plasmodium falciparum; RNA; Reproducibility of Results; Species Specificity; Urine
Bacterial Infections and Mycoses | Genetics and Genomics | Immunology and Infectious Disease | Medical Sciences | Public Health | Respiratory Tract Diseases
With rising rates of drug-resistant infections, there is a need for diagnostic methods that rapidly can detect the presence of pathogens and reveal their susceptibility to antibiotics. Here we propose an approach to diagnosing the presence and drug-susceptibility of infectious diseases based on direct detection of RNA from clinical samples. We demonstrate that species-specific RNA signatures can be used to identify a broad spectrum of infectious agents, including bacteria, viruses, yeast, and parasites. Moreover, we show that the behavior of a small set of bacterial transcripts after a brief antibiotic pulse can rapidly differentiate drug-susceptible and -resistant organisms and that these measurements can be made directly from clinical materials. Thus, transcriptional signatures could form the basis of a uniform diagnostic platform applicable across a broad range of infectious agents.
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Publisher PDF posted as allowed by the publisher's author rights policy at http://www.pnas.org/site/misc/authorfaq.shtml.
DOI of Published Version
Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6217-22. doi: 10.1073/pnas.1119540109. Epub 2012 Apr 2. Link to article on publisher's site
Proceedings of the National Academy of Sciences of the United States of America
Barczak AK, Gomez JE, Kaufmann BB, Hinson ER, Cosimi L, Borowsky ML, Onderdonk AB, Stanley SA, Kaur D, Bryant KF, Knipe DM, Sloutsky A, Hung DT. (2012). RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities. Center for Health Policy and Research (CHPR) Publications. https://doi.org/10.1073/pnas.1119540109. Retrieved from https://escholarship.umassmed.edu/healthpolicy_pp/86