Title
Bacteriophage P22 accessory recombination function
UMMS Affiliation
Graduate School of Biomedical Sciences; Graduate School of Biomedical Sciences; Department of Molecular Genetics and Microbiology
Publication Date
1991-05-01
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The accessory recombination function (arf) gene of bacteriophage P22 is located immediately upstream of the essential recombination function (erf) gene. Three mutant alleles of arf were constructed and installed in P22 in place of the wild-type allele: an out-of-frame internal deletion, an in-frame internal deletion, and an amber mutation. The deletion mutant phages are partially defective in homologous recombination and plaque formation in wild-type and recA hosts; their defects are more severe in recB and recA recB hosts. The amber mutant phage exhibits the same growth phenotypes in nonsuppressing hosts, but not in an amber-suppressor host. Plasmids that express arf complement the growth defect of arf- phages. These plasmids stimulate erf-mediated recombination; they were also found to cause a small stimulation of recA-recBCD-mediated homologous recombination of phage lambda.
Source
Virology. 1991 May;182(1):316-23.
Journal/Book/Conference Title
Virology
Related Resources
PubMed ID
1827223
Repository Citation
Poteete AR, Fenton AC, Semerjian A. (1991). Bacteriophage P22 accessory recombination function. GSBS Student Publications. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/999