DNA vaccines for influenza virus: differential effects of maternal antibody on immune responses to hemagglutinin and nucleoprotein
Graduate School of Biomedical Sciences; Program in Immunology and Virology; Division of Microbiology and Immunology
Medical Subject Headings
Animals; Animals, Newborn; Antibodies; Antibody Formation; Biolistics; CD8-Positive T-Lymphocytes; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Hemagglutinin Glycoproteins, Influenza Virus; Immunity, Cellular; *Immunity, Maternally-Acquired; Immunoglobulin G; Influenza A virus; Injections, Intramuscular; Interferon Type II; Mice; Mice, Inbred BALB C; Nucleoproteins; Orthomyxoviridae Infections; Pregnancy; Vaccines, DNA
Life Sciences | Medicine and Health Sciences
Maternal antibody is the major form of protection from disease in early life when the neonatal immune system is still immature; however, the presence of maternal antibody also interferes with active immunization, placing infants at risk for severe bacterial and viral infection. We tested the ability of intramuscular and gene gun immunization with DNA expressing influenza virus hemagglutinin (HA) and nucleoprotein (NP) to raise protective humoral and cellular responses in the presence or absence of maternal antibody. Neonatal mice born to influenza virus-immune mothers raised full antibody responses to NP but failed to generate antibody responses to HA. In contrast, the presence of maternal antibody did not affect the generation of long-lived CD8(+) T-cell responses to both HA and NP. Thus, maternal antibody did not affect cell-mediated responses but did affect humoral responses, with the ability to limit the antibody response correlating with whether the DNA-expressed immunogen was localized in the plasma membrane or within the cell.
Rights and Permissions
Citation: J Virol. 2000 Sep;74(17):7787-93.
Journal of virology
Pertmer, Tamera Marie; Oran, Alp E.; Moser, Janice M.; Madorin, Catherine A.; and Robinson, Harriet L., "DNA vaccines for influenza virus: differential effects of maternal antibody on immune responses to hemagglutinin and nucleoprotein" (2000). GSBS Student Publications. 981.