Title
Th1 genetic adjuvants modulate immune responses in neonates
Academic Program
Not applicable
UMMS Affiliation
Graduate School of Biomedical Sciences; Program in Immunology and Virology
Publication Date
2001-02-13
Document Type
Article
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
In these studies, we address the ability of DNA encoding Th1 cytokines to bias the isotype of antibody raised by neonatal or adult immunization with an influenza hemagglutinin expressing DNA (HA-DNA). Neonatal mice coimmunized with HA-DNA and either IL-12 or IFN-gamma-expressing DNA developed IgG2a-biased immune responses, regardless of inoculation method. In contrast, the Th1 genetic adjuvants had no effect on IgG subtype patterns in adults. In neonatal mice, the Th1 genetic adjuvants also shifted the pattern of lymphokine production by recall splenocytes from a mixed response of IFN-gamma and IL-5 to exclusively IFN-gamma. In adults, despite the failure to change the isotype pattern of the antibody response, a shift towards IFN-gamma production also occurred for recall splenocytes following coimmunzation with IL-12. Thus, coinoculation of Th1 genetic adjuvants had greater effects on the nature of the immune response in the neonate than in adults.
DOI of Published Version
10.1016/S0264-410X(00)00388-1
Source
Vaccine. 2001 Feb 8;19(13-14):1764-71.
Journal/Book/Conference Title
Vaccine
Related Resources
PubMed ID
11166902
Repository Citation
Pertmer TM, Oran AE, Madorin CA, Robinson HL. (2001). Th1 genetic adjuvants modulate immune responses in neonates. GSBS Student Publications. https://doi.org/10.1016/S0264-410X(00)00388-1. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/980