Genetic disassociation of autoimmunity and resistance to costimulation blockade-induced transplantation tolerance in nonobese diabetic mice
Graduate School of Biomedical Sciences; Program in Immunology and Virology; Department of Medicine, Diabetes Division; Department of Medicine, Division of Endocrinology and Metabolism; Program in Molecular Medicine
Medical Subject Headings
Animals; Antibodies, Monoclonal; Antigens, CD; Antigens, CD40; Antigens, CD86; Autoimmune Diseases; Bone Marrow Cells; CD4-CD8 Ratio; CD40 Ligand; CD8-Positive T-Lymphocytes; Cell Adhesion; Cell Count; Cell Differentiation; Cells, Cultured; Crosses, Genetic; Cytotoxicity, Immunologic; Dendritic Cells; Diabetes Mellitus, Type 1; Female; Genetic Markers; *Genetic Predisposition to Disease; Graft Survival; Homozygote; Immunity, Natural; Injections, Intravenous; Killer Cells, Natural; Lymphocyte Activation; Lymphocyte Transfusion; Lymphopenia; Macrophages; Male; Membrane Glycoproteins; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred NOD; Mice, Knockout; Receptors, Interleukin-2; Skin Transplantation; Transplantation Tolerance
Life Sciences | Medicine and Health Sciences
Curing type 1 diabetes by islet transplantation requires overcoming both allorejection and recurrent autoimmunity. This has been achieved with systemic immunosuppression, but tolerance induction would be preferable. Most islet allotransplant tolerance induction protocols have been tested in nonobese diabetic (NOD) mice, and most have failed. Failure has been attributed to the underlying autoimmunity, assuming that autoimmunity and resistance to transplantation tolerance have a common basis. Out of concern that NOD biology could be misleading in this regard, we tested the hypothesis that autoimmunity and resistance to transplantation tolerance in NOD mice are distinct phenotypes. Unexpectedly, we observed that (NOD x C57BL/6)F(1) mice, which have no diabetes, nonetheless resist prolongation of skin allografts by costimulation blockade. Further analyses revealed that the F(1) mice shared the dendritic cell maturation defects and abnormal CD4(+) T cell responses of the NOD but had lost its defects in macrophage maturation and NK cell activity. We conclude that resistance to allograft tolerance induction in the NOD mouse is not a direct consequence of overt autoimmunity and that autoimmunity and resistance to costimulation blockade-induced transplantation tolerance phenotypes in NOD mice can be dissociated genetically. The outcomes of tolerance induction protocols tested in NOD mice may not accurately predict outcomes in human subjects.
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Citation: J Immunol. 2003 Jul 1;171(1):185-95.
Journal of immunology (Baltimore, Md. : 1950)
Pearson, Todd; Markees, Thomas G.; Serreze, David V.; Pierce, Melissa A.; Marron, Michele P.; Wicker, Linda S.; Peterson, Laurence B.; Shultz, Leonard D.; Mordes, John P.; Rossini, Aldo A.; and Greiner, Dale L., "Genetic disassociation of autoimmunity and resistance to costimulation blockade-induced transplantation tolerance in nonobese diabetic mice" (2003). GSBS Student Publications. 972.