Retrograde transport by the microtubule-associated protein MAP 1C
Graduate School of Biomedical Sciences; Department of Cell Biology
Life Sciences | Medicine and Health Sciences
Microtubules are involved in several forms of intracellular motility, including mitosis and organelle movement. Fast axonal transport is a highly ordered form of organelle motility that operates in both the anterograde (outwards from the cell body) and retrograde (from the periphery towards the cell body) direction. Similar microtubule-associated movement is observed in non-neuronal cells, and might be involved in secretion, endocytosis and the positioning of organelles within the cell. Kinesin is a mechanochemical protein that produces force along microtubules in an anterograde direction. We recently found that the brain microtubule-associated protein MAP 1C (ref. 7) is a microtubule-activated ATPase and, like kinesin, can translocate microtubules in an in vitro assay for microtubule-associated motility. MAP 1C seemed to be related to the ciliary and flagellar ATPase, dynein, which is thought to produce force in a direction opposite to that observed for kinesin. Here we report that MAP 1C, in fact, acts in a direction opposite to kinesin, and has the properties of a retrograde translocator.
DOI of Published Version
Nature. 1987 Nov 12-18;330(6144):181-3. Link to article on publisher's site
Paschal BM, Vallee RB. (1987). Retrograde transport by the microtubule-associated protein MAP 1C. GSBS Student Publications. https://doi.org/10.1038/330181a0. Retrieved from https://escholarship.umassmed.edu/gsbs_sp/969