GSBS Student Publications
Title
A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Date
12-20-1988
Document Type
Article
Medical Subject Headings
*Chromosome Deletion; Chromosome Mapping; Chromosomes, Bacterial; Crosses, Genetic; *DNA Transposable Elements; Escherichia coli; *Genes; *Genes, Bacterial; Genotype; *Mutation; Site-Specific DNA-Methyltransferase (Adenine-Specific); Transduction, Genetic
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
We describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene (dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of E. coli.
Rights and Permissions
Citation: Gene. 1988 Dec 20;73(2):531-5.
Related Resources
Journal Title
Gene
PubMed ID
2854098
Repository Citation
Parker, Breck Olland and Marinus, Martin G., "A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation" (1988). GSBS Student Publications. 959.
https://escholarship.umassmed.edu/gsbs_sp/959