GSBS Student Publications

Title

The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells

Publication Date

2006-11-16

UMMS Affiliation

Graduate School of Biomedical Sciences; Department of Medicine; Program in Molecular Medicine

Document Type

Article

Disciplines

Life Sciences | Medicine and Health Sciences

Abstract

It has recently been suggested that the Saccharomyces cerevisiae protein Chl1p plays a role in cohesion establishment. Here, we show that the human ATP-dependent DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. Localization studies show that ChlR1 diffusely coats mitotic chromatin in prophase and then translocates from the chromatids to concentrate at the spindle poles during the transition to metaphase. Depletion of ChlR1 protein by RNA interference results in mitotic failure with replicated chromosomes failing to segregate after a pro-metaphase arrest. We show that depletion also results in abnormal sister chromatid cohesion, determined by increased separation of chromatid pairs at the centromere. Furthermore, biochemical studies show that ChlR1 is in complex with cohesin factors Scc1, Smc1 and Smc3. We conclude that human ChlR1 is required for sister chromatid cohesion and, hence, normal mitotic progression. These functions are important to maintain genetic fidelity.

DOI of Published Version

10.1242/jcs.03262

Source

J Cell Sci. 2006 Dec 1;119(Pt 23):4857-65. Epub 2006 Nov 14. Link to article on publisher's site

Journal/Book/Conference Title

Journal of cell science

Related Resources

Link to article in PubMed

PubMed ID

17105772

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