GSBS Student Publications
Title
Bipartite structure of the proximal promoter of a human H4 histone gene
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Cell Biology
Date
7-1-1995
Document Type
Article
Medical Subject Headings
Base Sequence; Binding Sites; Cross-Linking Reagents; DNA; Hela Cells; Histones; Humans; Molecular Sequence Data; Nuclear Proteins; *Promoter Regions (Genetics); Protein Binding; Ultraviolet Rays
Disciplines
Life Sciences | Medicine and Health Sciences
Abstract
The proximal promoter of the human H4 histone gene FO108 contains two regions of in vivo protein-DNA interaction, Sites I and II. Electrophoretic mobility shift assays using a radiolabeled DNA probe revealed that several proteins present in HeLa cell nuclear extracts bound specifically to Site I (nt-125 to nt-86). The most prominent complex, designated HiNF-C, and a complex of greater mobility, HiNF-C', were specifically compatable by an Sp1 consensus oligonucleotide. Fractionation of HiNF-C using wheat germ agglutinin affinity chromatography suggested that, like Sp1, HiNF-C contains N-acetylglucosamine moieties. Two minor complexes of even greater mobility, designated HiNF-E and F, were compatable by ATF consensus oligonucleotides. A DNA probe carrying a site-specific mutation in the distal portion of Site I failed to bind HiNF-E, indicating that this protein associated specifically to this region. UV cross-linking analysis showed that several proteins of different molecular weights interact specifically with Site I. These data indicate that Site I possesses a bipartite structure and that multiple proteins present in HeLa cell nuclear extracts interact specifically with Site I sequences.
Rights and Permissions
Citation: J Cell Biochem. 1995 Jul;58(3):372-9. Link to article on publisher's site
DOI of Published Version
10.1002/jcb.240580310
Related Resources
Journal Title
Journal of cellular biochemistry
PubMed ID
7593258
Repository Citation
Wright, Kenneth Lynn; Birnbaum, Mark J.; Van Wijnen, Andre J.; Stein, Gary S.; and Stein, Janet L., "Bipartite structure of the proximal promoter of a human H4 histone gene" (1995). GSBS Student Publications. 947.
https://escholarship.umassmed.edu/gsbs_sp/947